Integrative analysis of multi-omics datasets for Synechocystis sp. PCC 6803 identifies novel elements in the salt acclimation process of cyanobacteria

ABSTRACT: The salt acclimation process of the euryhaline model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by combining transcriptomic, proteomic and metabolomic methods. The comparison of salt-induced proteome and transcriptome changes revealed that most stably up-regulated proteins also showed elevated mRNA levels. The Pearson correlation coefficient for salt-induced abundance changes of 1749 transcript/protein pairs was r = 0.58. In addition to the rapid and stable upregulation of compatible solute biochemistry, a dynamic reorganization of the transcriptome occurred during the first hours after salt shock, which probably involves the action of small regulatory RNAs. Based on these data, an extended salt stimulon can be defined comprising many proteins directly or indirectly related to compatible solute metabolism, ion and water movements as well as a defined set of small regulatory RNAs. Our comprehensive data set provides the basis for future attempts to engineer cyanobacterial salt tolerance and to search for processes regulating this important environmental acclimation process.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Synechocystis Sp. (strain Pcc 6803 / Kazusa)

TISSUE(S): Photosynthetic Cell

SUBMITTER: Stefan Mikkat  

LAB HEAD: Martin Hagemann

PROVIDER: PXD026118 | Pride | 2022-02-17

REPOSITORIES: Pride