Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Physcomitrella Patens Subsp. Patens (moss)
TISSUE(S): Protonema
SUBMITTER: Ralf Reski
LAB HEAD: Ralf Reski
PROVIDER: PXD026651 | Pride | 2022-01-10
REPOSITORIES: Pride
Top Oguz O Milferstaedt Stella W L SWL van Gessel Nico N Hoernstein Sebastian N W SNW Özdemir Bugra B Decker Eva L EL Reski Ralf R
Communications biology 20210812 1
Production of biopharmaceuticals relies on the expression of mammalian cDNAs in host organisms. Here we show that the expression of a human cDNA in the moss Physcomitrium patens generates the expected full-length and four additional transcripts due to unexpected splicing. This mRNA splicing results in non-functional protein isoforms, cellular misallocation of the proteins and low product yields. We integrated these results together with the results of our analysis of all 32,926 protein-encoding ...[more]