Proteomics

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Characterization of waxy starches from CRISPR-Cas9 edited potato plants, the loss of GBSS1 influences the phosphate con-tent inside the starch granule

ABSTRACT: The granule-bound starch synthase is responsible for amylose biosynthesis. This enzyme is ac-tive when physically associated with starch granules where it represents up to 95% of starch-bound proteins. gbss1 mutants of Solanum tuberosum, cv Desiree were recently generated by CRIPR/Cas9-driven gene edition. Here, we investigated the impact of the modulation of GBSS1 contents on the starch-bound proteome and the resulting amylopectin structure. The total protein content in knock-out mutants was decreased by 50 %, which indicate a compensation by the other proteins since GBSS1 represent 85 % of the total protein content. This compensation was mainly due to the increase of starch-bound SS2 (starch synthase 2) and GWD (glucan water dikinase). These changes were accompanied by an increase of the phosphate content. Taken to-gether, these data reveal that GBSS1 loss is compensated by the association of other starch met-abolic enzymes with starch granules, with a strong increase of GWD, leading to modified amy-lopectin structure.

INSTRUMENT(S):

ORGANISM(S): Solanum Tuberosum (potato)

TISSUE(S): Tuber

SUBMITTER: BRAY FABRICE  

LAB HEAD: Nicolas Szydlowski

PROVIDER: PXD027193 | Pride | 2026-01-26

REPOSITORIES: Pride

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Loss of the granule-bound starch synthase protein reshapes the starch proteome and phosphorylation profile in potato tuber.

Helle Stanislas S   Sevestre François F   Veillet Florian F   Bray Fabrice F   Flament Stephanie S   Derbass Walaa W   Chauvin Laura L   Rolando Christian C   D'Hulst Christophe C   Chauvin Jean-Eric JE   Szydlowski Nicolas N  

International journal of biological macromolecules 20260116 Pt 2

Amylose and amylopectin define the structural behavior, gelatinization and retrogradation of starch, while starch phosphorylation modulates its hydration, viscosity, and stability. Granule-bound starch synthase (GBSS) is responsible for amylose biosynthesis, and phosphorylation is catalyzed by the granule associated glucan- and phosphoglucan- water dikinases, (GWD and PWD), respectively. To explore how GBSS influences the association of other starch-binding proteins, we analyzed the starch prote  ...[more]

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