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Xenopus Hwa IP-MS in 1 cell stage embryos

ABSTRACT: Spemann and Mangold Organizer (SMO) is of fundamental importance for the dorsal ventral body axis formation during vertebrate embryogenesis. Maternal Huluwa (Hwa) has been identified as the dorsal determinant that is both necessary and sufficient for the SMO formation. However, it remains unclear how Hwa is regulated. Here we report that the E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) is essential for restricting the spatial activity of Hwa, and therefore the proper SMO formation in Xenopus leavis. ZNRF3 interacts with and ubiquitinates Hwa thereby regulating its lysosomal trafficking and protein stability. Perturbation of ZNRF3 leads to the accumulation of Hwa and induction of ectopic axis in embryos. Ectopic expression of ZNRF3 promotes Hwa degradation and damps the axis-inducing activity of Hwa. Thus, our findings identify a substrate of ZNRF3, but also highlight the importance of the regulation of Hwa temporospatial activity in body axis formation in vertebrate embryos.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Embryo, Early Embryonic Cell

SUBMITTER: zhu xuechen

LAB HEAD: Tao Qinghua

PROVIDER: PXD027207 | Pride | 2021-09-29

REPOSITORIES: Pride

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