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Changes in Mycobacterium avium subsp. paratuberculosis proteome after cultivation in milk

ABSTRACT: Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne’s disease in cattle. MAP can be either shed directly into milk by infected cows, or introduced via fecal contamination. Viable MAP are detectable in milk and other dairy products, indicating survival of MAP after the pasteurization process. Although direct evidence is still lacking, MAP are discussed as a possible factor in the morbidity for chronic inflammatory bowel diseases in humans, such as Crohn’s disease and ulcerative colitis. Therefore, it is broadly accepted in the scientific community that exposure to MAP, especially through contaminated milk and dairy products, should be kept to a minimum. To gain deeper insight into the role of milk in MAP transmission and the question of why MAP can survive pasteurization, we investigated MAP proteome changes after incubation in milk at 37°C (simulating the environment in the mammary gland) and 4°C (simulating tank milk) as well as incubation in liquid control medium at 37°C.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine) Mycobacterium Avium Subsp. Paratuberculosis

TISSUE(S): Milk

SUBMITTER: Roxane Degroote

LAB HEAD: Stefanie M Hauck

PROVIDER: PXD027444 | Pride | 2021-08-26

REPOSITORIES: Pride

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