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SARS-CoV-2 variants of concern have acquired mutations associated with an increased spike cleavage

ABSTRACT: Quantification of Spike cleavage across different Sars-Cov2 variants by partial reaction monitoring mass spectrometry

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Covid-19

SUBMITTER: Andrea Fossati

LAB HEAD: Nevan Krogan

PROVIDER: PXD027641 | Pride | 2022-02-22

REPOSITORIES: Pride

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Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission.

Escalera Alba A Gonzalez-Reiche Ana S AS Aslam Sadaf S Mena Ignacio I Laporte Manon M Pearl Rebecca L RL Fossati Andrea A Rathnasinghe Raveen R Alshammary Hala H van de Guchte Adriana A Farrugia Keith K Qin Yiren Y Bouhaddou Mehdi M Kehrer Thomas T Zuliani-Alvarez Lorena L Meekins David A DA Balaraman Velmurugan V McDowell Chester C Richt Jürgen A JA Bajic Goran G Sordillo Emilia Mia EM Dejosez Marion M Zwaka Thomas P TP Krogan Nevan J NJ Simon Viviana V Albrecht Randy A RA van Bakel Harm H García-Sastre Adolfo A Aydillo Teresa T

Cell host & microbe 20220121 3

SARS-CoV-2 lineages have diverged into highly prevalent variants termed "variants of concern" (VOCs). Here, we characterized emerging SARS-CoV-2 spike polymorphisms in vitro and in vivo to understand their impact on transmissibility and virus pathogenicity and fitness. We demonstrate that the substitution S:655Y, represented in the gamma and omicron VOCs, enhances viral replication and spike protein cleavage. The S:655Y substitution was transmitted more efficiently than its ancestor S:655H in th ...[more]

PMID: 35150638

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