Systematic investigation of protein phosphorylation in biomolecular condensates

ABSTRACT: We have adapted a recently developed quantitative proteomics based approach to measure protein solubility following a lysate centrifugation assay to determine the proportion of a protein in distinct subpopulations in a proteome-wide manner (Sridharan et al., 2019). Further, by combining the solubility measurements with phosphoproteomics (Potel et al., 2021), we have quantitatively mapped phosphorylation sites enriched in the different protein subpopulations.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sindhuja Sridharan  

LAB HEAD: Mikhail M. Savitski

PROVIDER: PXD027769 | Pride | 2022-08-09

REPOSITORIES: Pride