Reversible Click Chemistry Tag for Universal Proteome Sample Preparation

ABSTRACT: Here, we introduce a reversible protein tag, ProMTag, that enables whole proteome capture, cleanup, and release of intact proteins for top-down analysis. Alternatively, the addition of a novel Trypsin derivative to the workflow generates peptides for bottom-up analysis. We show that the ProMTag workflow yields >90% for intact proteins and >85% for proteome digests. For top-down analysis, ProMTag cleanup improves resolution on 2D gels; for bottom-up exploration, this methodology produced reproducible mass spectrometry results, demonstrating that the ProMTag method is a truly universal approach that produces high quality proteome samples compatible with multiple downstream analytical techniques.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Gallus Gallus (chicken) Burkholderia Sp. 2332 Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Jonathan Minden  

LAB HEAD: Amber Lucas

PROVIDER: PXD027799 | Pride | 2022-02-14

REPOSITORIES: Pride