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Integrated analysis of the sialotrancriptome and sialoproteome of the rat flea Xenopsylla cheopis.

ABSTRACT: . Here we provide a deeper insight into Xenopsylla cheopis salivary glands contents pairing transcriptome and proteomic approaches. Sequencing of 99 pairs of salivary glands from adult females X. cheopis yielded a total of 7,432 coding sequences functionally classified into 25 classes, in which the secreted class was found to be the most abundant one. The translated transcripts also served as a reference database for the proteomic study, which identified peptides from 610 different proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Xenopsylla Cheopis

TISSUE(S): Salivary Gland

SUBMITTER: Stephen Lu

LAB HEAD: Jose Marcos Ribeiro

PROVIDER: PXD028695 | Pride | 2023-05-10

REPOSITORIES: Pride

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Integrated analysis of the sialotranscriptome and sialoproteome of the rat flea Xenopsylla cheopis.

Lu Stephen S Andersen John F JF Bosio Christopher F CF Hinnebusch B Joseph BJ Ribeiro José M C JMC

Journal of proteomics 20220104

Over the last 20 years, advances in sequencing technologies paired with biochemical and structural studies have shed light on the unique pharmacological arsenal produced by the salivary glands of hematophagous arthropods that can target host hemostasis and immune response, favoring blood acquisition and, in several cases, enhancing pathogen transmission. Here we provide a deeper insight into Xenopsylla cheopis salivary gland contents pairing transcriptomic and proteomic approaches. Sequencing of ...[more]

PMID: 34990822

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