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Identification of interactors of acetylated Ub

ABSTRACT: In this project we present the identification of interactors of mono-acetylated ubiquitin (Ub) variants from HEK293T whole cell extracts. Affinity enrichment coupled to high-resolution mass spectrometry followed by label-free quantification revealed the interactome of the respective mono-acetylated Ub variants.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Simon Kienle

LAB HEAD: Prof. Dr. Martin Scheffner

PROVIDER: PXD028797 | Pride | 2022-10-14

REPOSITORIES: Pride

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Electrostatic and steric effects underlie acetylation-induced changes in ubiquitin structure and function.

Kienle Simon Maria SM Schneider Tobias T Stuber Katrin K Globisch Christoph C Jansen Jasmin J Stengel Florian F Peter Christine C Marx Andreas A Kovermann Michael M Scheffner Martin M

Nature communications 20220916 1

Covalent attachment of ubiquitin (Ub) to proteins is a highly versatile posttranslational modification. Moreover, Ub is not only a modifier but itself is modified by phosphorylation and lysine acetylation. However, the functional consequences of Ub acetylation are poorly understood. By generation and comprehensive characterization of all seven possible mono-acetylated Ub variants, we show that each acetylation site has a particular impact on Ub structure. This is reflected in selective usage of ...[more]

PMID: 36114200

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Interactome of S65 phosphorylated NEDD8 and Ub

Project description:In this project we present for the first time a proteome-wide interaction study of S65 phosphorylated NEDD8 and ubiquitin (Ub). By using cell extracts and affinity enrichment coupled to mass spectrometry, we show that pNEDD8 and pUb have different interactomes that are also distinct from those of the non-modified forms. Among the preferential pNEDD8 interactors are HSP70 family members and, indeed, pNEDD8 stimulates HSP70 ATPase activity more pronouncedly than non-modified NEDD8. Our findings provide evidence that phosphorylation of Ub and NEDD8 at S65 is involved in stress response signaling and underscore the importance of studying non-covalent interactions of NEDD8 in particular and post-translational modifications of Ubls in general.

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Mouse embryonic kidneys (E13.5): mutant (UB HDAC1,2-/-) vs. wild type

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Project description:Here we defined changes in the levels of Ub-modified peptides upon rpn-6 knockdownthat in young C. elegans adults (day 5)

2021-05-26 | PXD024094 | Pride

Developmental proteomics of wild-type and K6W-Ub mouse congenital cataractous lenses

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