Dataset Information

Proteomic analysis of oil palm's response to red palm weevil infestation

ABSTRACT: Oil palm leaves were analysed via proteomics approach to identify the differentially-expressed proteins under Red Palm Weevil infestation on the first, third and sixth week post-infestation. The comparison was made among three groups; the control (C), physical wounding by drilling (wounded,W) and the red palm weevil larva infestation (Infested, I)

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Elaeis Guineensis Var. Tenera (oil Palm)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Nazmi Harith Fadzilah

LAB HEAD: Maizom Hassan

PROVIDER: PXD028986 | Pride | 2022-02-14

REPOSITORIES: Pride

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Publications

Proteomics and Interspecies Interaction Analysis Revealed Abscisic Acid Signalling to Be the Primary Driver for Oil Palm's Response against Red Palm Weevil Infestation.

Harith-Fadzilah Nazmi N Lam Su Datt SD Haris-Hussain Mohammad M Ghani Idris Abd IA Zainal Zamri Z Jalinas Johari J Hassan Maizom M

Plants (Basel, Switzerland) 20211125 12

The red palm weevil (RPW; <i>Rhynchophorus ferrugineus</i> Olivier (Coleoptera Curculionidae)) is an invasive insect pest that is difficult to manage due to its nature of infesting the host palm trees from within. A holistic, molecular-based approach to identify proteins that correlate with RPW infestation could give useful insights into the vital processes that are prevalent to the host's infestation response and identify the potential biomarkers for an early detection technique. Here, a shotgu ...[more]

PMID: 34961045

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Project description:BACKGROUND: The tomato psyllid, Bactericera cockerelli Šulc (Hemiptera: Triozidae), is a pest of solanaceous crops such as tomato (Solanum lycopersicum L.) in the U.S. and vectors the disease-causing pathogen ‘Candidatus Liberibacter solanacearum’. Currently, the only effective strategies for controlling the diseases associated with this pathogen involve regular pesticide applications to manage psyllid population density. However, such practices are unsustainable and will eventually lead to widespread pesticide resistance in psyllids. Therefore, new control strategies must be developed to increase host-plant resistance to insect vectors. For example, expression of constitutive and inducible plant defenses can be improved through selection. Currently, it is still unknown whether psyllid infestation has any lasting consequences on tomato plant defense or tomato plant gene expression in general. RESULTS: To characterize the genes putatively involved in tomato defense against psyllid infestation, RNA was extracted from psyllid-infested and uninfested tomato leaves (Moneymaker) three weeks post-infestation. Transcriptome analysis identified 362 differentially expressed genes. These differentially expressed genes were primarily associated with defense responses to abiotic/biotic stress, transcription/translation, cellular signaling/transport, and photosynthesis. These gene expression changes suggested that tomato plants underwent a reduction in plant growth/health in exchange for improved defense against stress that was observable three weeks after psyllid infestation. Consistent with these observations, tomato plant growth experiments determined that the plants were shorter three weeks after psyllid infestation. Furthermore, psyllid nymphs had lower survival rates on tomato plants that had been previously psyllid infested. CONCLUSION: These results suggested that psyllid infestation has lasting consequences for tomato gene expression, defense, and growth.

Dataset Information

Proteomic analysis of oil palm's response to red palm weevil infestation

Publications

Proteomics and Interspecies Interaction Analysis Revealed Abscisic Acid Signalling to Be the Primary Driver for Oil Palm's Response against Red Palm Weevil Infestation.

Similar Datasets

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