Dataset Information

Intracellular and extracellular proteome characterization of virulent and avirulent Bursaphelenchus xylophilus isolates

ABSTRACT: The pinewood nematode (PWN), Bursaphelenchus xylophilu (Bx) s, one of the most serious forest pests, worldwide, is considered the causal agent of the pine wilt disease (PWD). The main host species belong to the genus Pinus and a variation in the susceptibility of several pine species to PWN infection is well-known. Additionally, it is also recognized that there is variation in the virulence among different B. xylophilus isolates. In the present study, we applied a quantitative mass spectrometry-based proteomics approach to perform a deep characterization of proteomic changes across two B. xylophilus isolates from different hosts and geographical origins and with different virulence. A total of 1456 proteins were quantified and compared between the two isolates secretomes and a total of 2741 proteins were quantified and compared between the nematode proteomes in two different conditions, pine tree extract and fungus stimuli.

INSTRUMENT(S):

ORGANISM(S): Cellular Organisms Bursaphelenchus Xylophilus (pinewood Nematode Worm) (aphelenchoides Xylophilus)

TISSUE(S): Organism Form, Secretion By Cell

SUBMITTER: Sandra Anjo

LAB HEAD: Joana Sá

PROVIDER: PXD029377 | Pride | 2022-01-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BX_PRJEA64437_WBPS11_IS.fasta	Fasta
	S190505_BxAv_Fungus_1.wiff	Wiff
	S190505_BxAv_Fungus_1.wiff.scan	Wiff
	S190505_BxAv_Fungus_2.wiff	Wiff
	S190505_BxAv_Fungus_2.wiff.scan	Wiff

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Publications

Virulence Biomarkers of Bursaphelenchus xylophilus: A Proteomic Approach.

Cardoso Joana M S JMS Anjo Sandra I SI Manadas Bruno B Silva Hugo H Abrantes Isabel I Nakamura Katsunori K Fonseca Luís L

Frontiers in plant science 20220208

The pinewood nematode (PWN), Bursaphelenchus xylophilus, one of the most serious forest pests worldwide, is considered the causal agent of the pine wilt disease (PWD). The main host species belong to the genus Pinus, and a variation in the susceptibility of several pine species to PWN infection is well-known. It is also recognized that there is variation in the virulence among B. xylophilus isolates. In the present study, we applied a quantitative mass spectrometry-based pro ...[more]

PMID: 35211137

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Project description:The significant morphological differences observed during embryo development in angiosperms and gymnosperms are expected to be the result of a differential control of gene expression. We used a loblolly pine (Pinus taeda) cDNA microarray to analyze global transcriptional changes along zygotic embryogenesis in maritime pine (Pinus pinaster). A time-course analysis of the data obtained from the five embryo developmental groups used in this study led to the identification of 4,645 genes whose expression varied along P. pinaster embryogenesis. These transcripts were clustered into six distinct expression profiles. The grouping of these profiles in early, mid-embryogenesis and embryo maturation, according to the developmental period where most of the sequences were up-regulated, evidenced that characteristic transcriptional changes are associated to each developmental period. The application of a cut-off value of 1.95-fold change led to the identification of 1,838 differentially expressed transcripts that were categorized by biological process. Metabolism, interaction with the environment, oxidation-reduction and transport were some of the most represented categories. During early embryogenesis genes putatively involved in phytohormone-mediated signaling were identified, whereas in middle stages the overrepresented genes could be associated with cotyledon formation and induction of somatic embryogenesis. Genes associated with the synthesis of storage products were up-regulated in the latest stages of pine embryo development. It was also during this developmental period that the largest number of sequences putatively encoding transcription factors was identified. Pine immature zygotic embryos were pooled into five different developmental groups according to the collection date (Day0; Day5; Day11; Day15 and Day25). A common reference design was used. Total RNA was extracted from each sample. Three extractions were prepared per sample (biological replicates). A defined ammount from each RNA sample was pooled to use as reference. Messenger RNA was amplified for both test samples and reference. These were hybridized together in two separate experiments (technical replicates). In total, 30 slides were analyzed.

Dataset Information

Intracellular and extracellular proteome characterization of virulent and avirulent Bursaphelenchus xylophilus isolates

Dataset's files

Publications

Virulence Biomarkers of <i>Bursaphelenchus xylophilus</i>: A Proteomic Approach.

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