Proteomics

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Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence


ABSTRACT: This investigation reports a differential proteomic analysis of the secretome of six S. aureus strains belonging to two genotypes with opposite within-herd prevalence, GTB (high) and GTS (low), corresponding to sequence types (ST) 8 and 398, respectively. Total proteins released in the growth medium were subjected to high-resolution tandem mass spectrometry and differential analysis with Proteome Discoverer by label-free approach. Here, we reported both the characterization of secretome proteins and a panel of differential proteins specific of S. Aureus depending on high or low within-herd prevalence. GTB/ST8 (High) released more immunoglobulin-binding proteins, complement and antimicrobial peptide inhibitors, enterotoxins, and metabolic enzymes, while GTS/ST398 (Low) released more leukocidins, hemolysins, lipases, and peptidases. Furthermore, GTB/ST8 (High) released the von Willebrand factor protein, staphylokinase, and clumping factor B, while GTS/ST398 (Low) released the staphylococcal coagulase and clumping factor A. In conclusion, our results provide an in-depth characterization of secretome proteins of the three S. aureus GTB/ST8 and three GTS/ST398 strains with high and low within-herd prevalence. We describe the role of extracellular proteins in S. Aureus pathogenesis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus

TISSUE(S): Cell Suspension Culture

SUBMITTER: Salvatore Pisanu  

LAB HEAD: Maria Filippa Addis

PROVIDER: PXD029571 | Pride | 2022-02-14

REPOSITORIES: Pride

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Publications

Comparative secretome analysis of <i>Staphylococcus aureus</i> strains with different within-herd intramammary infection prevalence.

Addis M Filippa MF   Pisanu Salvatore S   Monistero Valentina V   Gazzola Alessandra A   Penati Martina M   Filipe Joel J   Di Mauro Susanna S   Cremonesi Paola P   Castiglioni Bianca B   Moroni Paolo P   Pagnozzi Daniela D   Tola Sebastiana S   Piccinini Renata R  

Virulence 20221201 1


<i>Staphylococcus aureus</i> is a major pathogen causing intramammary infection and mastitis in dairy cows. <i>S. aureus</i> genotypes (GT) can differ significantly in their ability to diffuse and persist in the herd; while the association of virulence gene carriage with epidemiological behavior remains unclear, a role for secreted proteins has been postulated. We characterized the secretome of six <i>S. aureus</i> strains belonging to two genotypes with opposite within-herd prevalence, GTB (hig  ...[more]

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