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Quantitative acetylome analysis of Fusarium oxysporum

ABSTRACT: Quantitative proteome and acetylome analyses were conducted by comparing the acetylation levels of the SM to those of the NM. The collected cellular proteins were extracted, digested, and labeled by different TMTs. For acetylation profiling, the pan acetyl-lysine antibody was employed to enrich the acetylated peptides, which were then submitted into mass spectrometer (MS) for LC–MS/MS analysis. For proteome profiling, the peptide fractions were submitted into MS directly.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Fusarium Cf. Oxysporum

TISSUE(S): Mycelium

SUBMITTER: Ning Zhang

LAB HEAD: Ning Zhang

PROVIDER: PXD029583 | Pride | 2022-06-09

REPOSITORIES: Pride

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Quantitative Proteomic Analysis Reveals Important Roles of the Acetylation of ER-Resident Molecular Chaperones for Conidiation in Fusarium oxysporum.

Lv Fangjiao F Xu Yang Y Gabriel Dean W DW Wang Xue X Zhang Ning N Liang Wenxing W

Molecular & cellular proteomics : MCP 20220408 5

Fusarium oxysporum is one of the most abundant and diverse fungal species found in soils and includes nonpathogenic, endophytic, and pathogenic strains affecting a broad range of plant and animal hosts. Conidiation is the major mode of reproduction in many filamentous fungi, but the regulation of this process is largely unknown. Lysine acetylation (Kac) is an evolutionarily conserved and widespread posttranslational modification implicated in regulation of multiple metabolic processes. A total o ...[more]

PMID: 35398590

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