Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and their adaptation to different salinity probed by a proteomic approach

ABSTRACT: Osmotic changes are common challenges for marine microorganisms. Bacteria developed numerous ways of dealing with this stress, including reprogramming of global cellular processes, however, many molecular details were obtained only for the model bacteria. In this work we asked what is the basis of the adjustment to prolonged salinity challenges at the proteome level. The objects of our studies were three representatives of bacteria inhabiting various marine environments, Shewanella baltica, Vibrio harveyi and Aliivibrio fischeri. The proteomic studies were performed with bacteria cultivated in increased and decreased salinity, followed by proteolytic digestion of samples which were then subjected to liquid chromatography with tandem mass spectrometry analysis. We show that bacteria adjust at all levels of their biological processes, from DNA topology through gene expression regulation and proteasome assembly, to transport and cellular metabolism. Finding that many similar adaptation strategies were observed for both, low and high salinity conditions, is particularly interesting. The results show that adaptation to salinity challenge involves accumulation of DNA-binding proteins and increased polyamine uptake, and we hypothesize that their function is to coat and protect the nucleoid to counteract adverse changes in the DNA topology due to ionic shifts.

INSTRUMENT(S):

ORGANISM(S): Aliivibrio Fischeri Shewanella Baltica Vibrio Harveyi

SUBMITTER: Paulina Czaplewska  

LAB HEAD: Paulina Czaplewska

PROVIDER: PXD029628 | Pride | 2022-02-17

REPOSITORIES: Pride