Proteomics

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DIA Based quantitative proteomics analysis reveals MAPK pathway a important role onthe Tremella fuciformis dimorphism

ABSTRACT: Tremella fuciformis is a dimorphic fungus that can undertake the reversible transition between yeast-like spores and hypha forms. In this project, we attempted to explore the differential proteins profile of dikaryotic yeast-like spores (FBMDS) and dikaryotic mycelium (DM) by applying the HRMS1-DIA full proteomics and PRM target proteomics synthetically. The results showed that a total of 5687 proteins were quantified, and 2220 of them (39.01%) showed more than a two-fold change in expression. The functional analysis of differential expression proteins (DEPs) confirmed that the DEPs were mainly located on membrane and nucleus, and FBMDS tended to express proteins involved in DNA replication and transcription, DNA damage repair, biosynthesis, and metabolism. At the same time, DM exhibited increased expression of signal transduction such as MAPK signaling pathway, Ras signaling pathway.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Tremella

TISSUE(S): Cell Culture

SUBMITTER: Li Yaxing  

LAB HEAD: Sun Shujing

PROVIDER: PXD029989 | Pride | 2022-04-04

REPOSITORIES: Pride

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Publications

Study of Dimorphism Transition Mechanism of <i>Tremella fuciformis</i> Based on Comparative Proteomics.

Li Yaxing Y   Tang Haohao H   Zhao Weichao W   Yang Yang Y   Fan Xiaolu X   Zhan Guanping G   Li Jiahuan J   Sun Shujing S  

Journal of fungi (Basel, Switzerland) 20220228 3

<i>Tremella fuciformis</i> is a dimorphic fungus that can undertake a reversible transition between yeast-like conidia and hyphal forms. The transformation mechanism and proteomic differences between these two forms have not been reported. Therefore, in this study, we attempted to explore the differential protein profiles of dikaryotic yeast-like conidia from fruiting bodies and mycelia (FBMds) and dikaryotic mycelia (DM) by synthetically applying high-resolution MS1-based quantitative data-inde  ...[more]

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