Dataset Information

Discrimination of Tilletia controversa from the T. caries / T. laevis complex by MALDI-TOF MS analysis of teliospores

ABSTRACT: It is of high importance to distinguish Tilletia caries and Tilletia laevis as causal agents of common bunt accurately from Tilletia controversa, the causal agent of dwarf bunt. All three of these wheat bunt diseases can lead to significant yield losses in crop production worldwide. But T. controversa is categorized as a quarantine pest in most areas of the world and must be discriminated from the T. caries / T. laevis complex. Usually, morphological characteristics of the teliospores are used to differentiate the three species. But due to natural hybridization and overlapping properties the discrimination is challenging. Germination behavior can also be considered for discrimination, but equivalent to their similar physiological and genetic traits the two agents of common bunt, T. caries and T. laevis could not be distinguished by this. It was suggested that the two species and maybe all three of those described Tilletia species might be conspecific. Up to now no molecular based method is available to differentiate the three species. Several studies have attempted the detection of the wheat bunt Tilletia species using PCR or other DNA-based methods. Other studies analyzed protein patterns with electrophoresis methods. But none of these approaches was able to distinguish between all of the three closely related Tilletia species. Several studies have shown that Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) is a useful tool to differentiate closely related fungal species. The aim of this study was to assess whether MALDI-TOF MS analysis is able to distinguish specimens of the three closely related pathogens T. caries, T. laevis, and T. controversa and may constitute an alternative method to the usually used morphology-based identification. Therefore MALDI-TOF MS was used to create subproteome fingerprints of the teliospores of 69 Tilletia specimens. These fingerprints were analyzed by comparing the mass spectra to each other by high-throughput multidimensional scaling (HiT-MDS ) together with hierarchical cluster analysis (HCA). The second approach was performed by discriminant analysis of principal components (DAPC). MALDI-TOF MS has proven to be a useful method for distinguishing between T. controversa and the two causal agents of common bunt, using our developed method of direct analysis of teliospores, but was unable to separate T. caries and T. laevis species. We conclude a potentially conspecific status of T. caries and T. laevis or even two morphotypes of one common species, causing identical disease symptoms and sharing the same germination requirements along with a related protein composition, shown in this study. Our developed MALDI-TOF MS method can be helpful in testing Tilletia bunt balls collected during field inspections, especially with regard to quarantine regulations or for breeding applications and may also be transferred to analyze further challenging sample material.

INSTRUMENT(S): microflex LT

ORGANISM(S): Tilletia Controversa Tilletia Indica Tilletia Laevis Tilletia Caries

TISSUE(S): Basidiospore

SUBMITTER: Monika Forster

LAB HEAD: Niessen Ludwig Martin

PROVIDER: PXD030401 | Pride | 2022-02-14

REPOSITORIES: Pride

ACCESS DATA

Publications

Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores.

Forster Monika K MK Sedaghatjoo Somayyeh S Maier Wolfgang W Killermann Berta B Niessen Ludwig L

Applied microbiology and biotechnology 20220117 3

The fungal genus Tilletia includes a large number of plant pathogens of Poaceae. Only a few of those cause bunt of wheat, but these species can lead to significant yield losses in crop production worldwide. Due to quarantine regulations and specific disease control using appropriate seed treatments for the different disease agents, it is of high importance to distinguish Tilletia caries and Tilletia laevis as causal agents of common bunt accurately from Tilletia controversa, the causal agent of ...[more]

PMID: 35037998

Similar Datasets

Project description:The lesions of enamel caries can be considered as the outcome of dysbiotic changes in the biofilm community of supragingival dental plaque. Demineralization occurs as the cumulative outcome of repeated shifts towards a less diverse microbiota that produces and tolerates a low pH environment in tooth sites that are sheltered from protective factors in host saliva. Although the etiology of caries is multifactorial, frequent consumption of foods rich in fermentable carbohydrates, notably sucrose, appears to be one of the major factors driving the microbiota in the direction of dysbiosis, particularly in the case of otherwise healthy children with normal salivary flow. Streptococcus mutans and closely related species (such as Streptococcus sobrinus) have long been considered to play a primary etiological role in dental caries. S. mutans responds to sucrose by producing large quantities of lactic acid. It is very tolerant of low pH, and produces an insoluble extracellular polysaccharide that may sequester acid at tooth surfaces. The mechanisms behind those putative virulence factors have been intensively studied in monoculture, and recently in simple multi-species consortia. Much less is known of other species that may also contribute to or protect against dysbiosis driven by dietary carbohydrates. Some strains of “non-mutans” streptococci produce and tolerate acid at levels comparable to S. mutans, while others show increased ariginolytic capabilities, which may act to raise pH within the biofilm matrix. S. mutans tends to be a minority species even in caries-active children, and carious lesions likewise can occur in children with no detectable S. mutans. 16S rDNA-based metagenomic comparisons of caries-active and caries-free subjects have detected associations between caries and a variety of oral species, including not only non-mutans streptococci, but also members of other genera, such as Scardovia and Bifidobacterium. Caries associations have not been consistent between studies. Moreover, different taxonomic clusters have been defined as subgroups within the same study. This raises an important point. Although caries-associated communities are typically less diverse than healthy supragingival plaque overall, those dysbiotic communities still display considerable taxonomic diversity between affected individuals. That in turn raises the question of whether it is desirable to define biomarkers of dysbiosis that are less dependent on taxonomy. The Human Microbiome project generated comprehensive metagenomic data for a wide variety of body sites in healthy subjects, including supragingival plaque. Although most of that data was based on 16S rDNA sequencing, shotgun metagenomics was also used to catalog the functional potential of all microbial genes within a smaller subset of subjects. One of the key findings was that healthy sites from different people were broadly similar with respect to their functional profiles, even though there was extensive individual variation in their taxonomic profiles. It is possible that the “conservation of function” concept may also extend to dysbiotic communities. This would explain why microbial communities associated with caries still show considerable taxonomic variation. In that case, differential patterns of community-wide gene and/or protein expression might provide a more accurate indicator of dysbiosis than can be achieved by counting caries-associated species. Metatranscriptomic or metaproteomic approaches can be used to provide information on function. A recent metatranscriptomic comparison of subgingival plaque from healthy and periodontally diseased sites in three subjects has provided data that support the “conservation of function” concept. They observed that taxonomically diverse diseased sites shared conserved gene expression profiles [20]. By the same token, a recent metaproteomic comparison of gut microbiotas from healthy controls to Crohn’s disease patients found that major shifts in protein expression by function did not always correlate with changes in taxon relative abundance [21]. In this metaproteomic study, we found that sucrose–induced changes in protein expression patterns for pathways involving glycolysis, lactate production, aciduricity and ammonia/glutamate metabolism were likewise conserved in taxonomically diverse dysbiotic oral microcosm biofilm communities.

Dataset Information

Discrimination of Tilletia controversa from the T. caries / T. laevis complex by MALDI-TOF MS analysis of teliospores

Publications

Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure