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Label-free quantitative proteome of CD34+ or CKIT+ AML cells and mobilized PB CD34+ cells

ABSTRACT: The label-free quantitative proteome was generated for 30 primary AML patient samples enriched for CD34+ cells or CKIT+ cells in the case of NPMcyt samples. As controls 3 mobilized peripheral blood CD34+ cells were included.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Bone Marrow, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: Jan Jacob Schuringa

LAB HEAD: Jan Jacob Schuringa

PROVIDER: PXD030487 | Pride | 2025-01-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Sample_and_Data_relationship_Format_SDRF_file.txt	Txt
	Schuringa_AML_proteome_normalized_dataset.xlsx	Xlsx
	Schuringa_AML_proteome_result_intensities_output_2020.xlsx	Xlsx

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Publications

Bleximenib, the novel menin-KMT2A inhibitor JNJ-75276617, impairs long-term proliferation and immune evasion in acute myeloid leukemia.

Hogeling Shanna M SM Lê Duy Minh DM La Rose Nikita N Kwon Min Chul MC Wierenga Albertus T J ATJ Van den Heuvel Fiona A J FAJ Van den Boom Vincent V Kuchnio Anna A Philippar Ulrike U Huls Gerwin G Schuringa Jan Jacob JJ

Haematologica 20241219 6

Acute myeloid leukemia (AML) remains challenging to treat, which in part relates to genetic heterogeneity of the disease, to the protective tumor microenvironment driving resistance to therapy, and also to immune evasion characteristics of leukemic cells. Targeting epigenetic programs in AML provides an attractive opportunity to impair long-term proliferation and induce differentiation. The novel inhibitor JNJ-75276617 (bleximenib) targets the menin-KMT2A interaction and has shown preclinical ef ...[more]

PMID: 39704147

Publication: 1/2

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Label-free quantitative proteome of AML CD34+ and mobilized PB CD34+ cells

Project description:The label-free quantitative proteome was generated for 42 primary AML patient samples enriched for CD34+ cells (or mononuclear cells in the case of NPMcyt sameples) and as controls 6 mobilized peripheral blood CD34+ cells were included. Furthermore, 6 AML cell lines were included, and also primary mesenchymal stem cells grown under normaoxia or hypoxia were included.

2022-03-15 | PXD030463 | Pride

Transcriptome Study of CD34+ hematopoietic progenitor from patients reached of Primary Myelofibrosis

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Transcription profiling of mouse HSC(CD34-KSL cells) and progenitors (CD34+KSL cells)

Project description:Gene expression profile between HSC(CD34-KSL cells) and Progenitors(CD34+KSL cells) were compared.

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Characterization of human CD34+ HSC-derived neutrophils without myeloid-derived immunosuppressive cell activity

Project description:Neutrophils have limited utility as transfusion product due to their short lifespan. Culturing neutrophils from stem cell sources holds potential for fundamental and translational purposes. Here, we cultured CD34+ hematopoietic stem cell-derived neutrophils and compared them with peripheral blood neutrophils in terms of morphology, phenotype, and function. Our culture system resulted in 60% morphologically mature CD15+CD11b+CD16high neutrophils with most effector functions almost indistinguishable from blood neutrophils, confirmed by a high similarity in transcription and protein abundance patterns. While exhibiting strong microbial killing capacity and antibody-dependent cellular cytotoxicity against tumor cells, these cells were deficient in myeloid-derived suppressor cell activity. Upon dissecting the underlying mechanism, this deficiency in immunosuppressive activity correlated with their distinct granular composition in comparison to blood neutrophils. Taken together, our cultured neutrophils closely resemble blood neutrophils, offering a repository for fundamental research and a step towards an effective transfusion product without immunosuppressive activity.

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Transcription profiling of human CD34+ cells and treatment with UTP and CXCL12

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