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Protein translation upon DNA damage

ABSTRACT: LC-MS/MS analyses were performed in vehicle- and camptothecin (CPT)-treated 293T cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ruyuan Yu

LAB HEAD: Ruyuan Yu

PROVIDER: PXD031047 | Pride | 2022-08-26

REPOSITORIES: Pride

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LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms.

Yu Ruyuan R Hu Yameng Y Zhang Shuxia S Li Xincheng X Tang Miaoling M Yang Meisongzhu M Wu Xingui X Li Ziwen Z Liao Xinyi X Xu Yingru Y Li Man M Chen Suwen S Qian Wanying W Gong Li-Yun LY Song Libing L Li Jun J

Nucleic acids research 20220801 14

Sustaining DNA damage response (DDR) signalling via retention of DDR factors at damaged sites is important for transmitting damage-sensing and repair signals. Herein, we found that DNA damage provoked the association of ribosomes with IRES region in lncRNA CTBP1-DT, which overcame the negative effect of upstream open reading frames (uORFs), and elicited the novel microprotein DNA damage-upregulated protein (DDUP) translation via a cap-independent translation mechanism. Activated ATR kinase-media ...[more]

PMID: 35849344

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Transcriptional profiling of 293T cells treated with 2µM camptothecin compared to untreated cells

Project description:Total RNA from untreated 293T cells (2 samples) and 293T cells treated with 2µM camptothecin for 48h (2 samples) was isolated using RNeasy Midi Kit (QIAGEN, Valencia) followed by gene expression profiling on Affymetrix human U133A arrays and analysis by MAS 5.1. Keywords = 293T cells Keywords = camptothecin Keywords: parallel sample

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Untreated vs. Camptothecin Treated HeLa cells

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Transcription profiling of human Hela cells treated with the topoisomerase poison camptothecin

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