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Mouse inner organ injury Peritomeum, Liver, Cecum

ABSTRACT: Internal organs heal injuries with new connective tissue. However the cellular and molecular events, and the sources of this tissue, remain obscure. Here we tagged extracellular matrix around the mesothelium lining various mouse tissues: peritoneum, liver, and cecum, and applied various injury models. We discovered that preexisting matrix is transferred across organs into wounds. Using proteomics, genetic lineage-tracing and by selectively injuring juxtaposed organs, we demonstrate that the matrix tissue of origin likely dictates the final healing outcome: whether scarring or regeneration.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver, Cecum, Peritoneum

DISEASE(S): Wounds And Injuries

SUBMITTER: Safouane Kadri

LAB HEAD: Dr. Yuval Rinkevich

PROVIDER: PXD031766 | Pride | 2022-05-20

REPOSITORIES: Pride

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Neutrophils direct preexisting matrix to initiate repair in damaged tissues.

Fischer Adrian A Wannemacher Juliane J Christ Simon S Koopmans Tim T Kadri Safwen S Zhao Jiakuan J Gouda Mahesh M Ye Haifeng H Mück-Häusl Martin M Krenn Peter W PW Machens Hans-Günther HG Fässler Reinhard R Neumann Philipp-Alexander PA Hauck Stefanie M SM Rinkevich Yuval Y

Nature immunology 20220330 4

Internal organs heal injuries with new connective tissue, but the cellular and molecular events of this process remain obscure. By tagging extracellular matrix around the mesothelium lining in mouse peritoneum, liver and cecum, here we show that preexisting matrix was transferred across organs into wounds in various injury models. Using proteomics, genetic lineage-tracing and selective injury in juxtaposed organs, we found that the tissue of origin for the transferred matrix likely dictated the ...[more]

PMID: 35354953

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