Proteomics

Dataset Information

0

S-nitrosylation of p53 in A375 cells


ABSTRACT: Nitric oxide (NO) is a pleiotropic signaling molecule that affects numerous biological functions. One unique posttranslational modification of proteins by NO is termed S-nitrosylation (SNO), but the role in modulating key proteins’ function in melanoma has not been fully characterized. In the present study, by conducting the biotin switch assay and mass spectrometry, we confirmed that p53 was S-nitrosylated under nitrosative stress. Further proteomic characterization showed that Cys242, Cys275, and Cys277 were S-nitrosylated in A375 cells treated with 100 µM GSNO.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Cutaneous Malignant Melanoma 1

SUBMITTER: Yong Qin  

LAB HEAD: Yong Qin

PROVIDER: PXD031807 | Pride | 2025-07-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P53.RAW Raw
P53.dat Other
P53.mgf Mgf
P53.mzid Mzid
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Publications

S-Nitrosylation of p53 in Melanoma Cells Under Nitrosative Stress.

Grigoruta Mariana M   Li Li L   Chen Leyuan L   Meng Fancui F   Rosenblatt Kevin P KP   Li Yiliang Y   Grimm Elizabeth A EA   Qin Yong Y  

International journal of molecular sciences 20250706 13


Article [...]. ...[more]

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