Proteomics

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Evaluation of transgenic proteins in transformed Camelina sativa leaves


ABSTRACT: The goal of this study was to detect and quantify the presence of transgenic proteins in transformed C. sativa plants. Experimental plants were transformed with recombinant proteins of a synthetic carbon fixation cycle. Plants expressing the full suite of genes of the synthetic cycle are labeled as “T”. Plants expressing only half of the synthetic cycle are labeled as “C”. Plants transformed with an “empty vector” are labeled as “EV” and serve as a negative control. Five biological replicates were included for each treatment. Additionally, one chloroplast enrichment sample was generated for each line using a commercially available chloroplast isolation kit (Sigma Product #: CPISO). Leaf tissue from photosynthetically-active leaves was harvested and immediately flash frozen with liquid nitrogen. This tissue was stored at -80ºC until it was ground to a fine powder under liquid nitrogen. The tissue was then weighed into 200 mg aliquots. Amino acid sequences of the transgenic proteins were used as a reference and “empty vector” control plants were used as a negative control.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Camelina Sativa (false Flax) (myagrum Sativum)

TISSUE(S): Photosynthetic Cell, Leaf

SUBMITTER: Simina Vintila  

LAB HEAD: Manuel Kleiner

PROVIDER: PXD031909 | Pride | 2025-06-30

REPOSITORIES: Pride

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Publications

<i>In vitro</i> demonstration and <i>in planta</i> characterization of a condensed, reverse TCA (crTCA) cycle.

Wilson Nathan N   Smith-Moore Caroline C   Xu Yuan Y   Edwards Brianne B   La Hovary Christophe C   Li Kai K   Aslett Denise D   Ji Mikyoung M   Lin Xuli X   Vintila Simina S   Kleiner Manuel M   Xie Deyu D   Shachar-Hill Yair Y   Grunden Amy A   Sederoff Heike H  

Frontiers in plant science 20250609


<h4>Introduction</h4>Plants employ the Calvin-Benson cycle (CBC) to fix atmospheric CO<sub>2</sub> for the production of biomass. The flux of carbon through the CBC is limited by the activity and selectivity of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO). Alternative CO<sub>2</sub> fixation pathways that do not use RuBisCO to fix CO<sub>2</sub> have evolved in some anaerobic, autotrophic microorganisms.<h4>Methods</h4>Rather than modifying existing routes of carbon metabolism in pl  ...[more]

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