Evaluation of transgenic proteins in transformed Camelina sativa leaves

ABSTRACT: The goal of this study was to detect and quantify the presence of transgenic proteins in transformed C. sativa plants. Experimental plants were transformed with recombinant proteins of a synthetic carbon fixation cycle. Plants expressing the full suite of genes of the synthetic cycle are labeled as “T”. Plants expressing only half of the synthetic cycle are labeled as “C”. Plants transformed with an “empty vector” are labeled as “EV” and serve as a negative control. Five biological replicates were included for each treatment. Additionally, one chloroplast enrichment sample was generated for each line using a commercially available chloroplast isolation kit (Sigma Product #: CPISO). Leaf tissue from photosynthetically-active leaves was harvested and immediately flash frozen with liquid nitrogen. This tissue was stored at -80ºC until it was ground to a fine powder under liquid nitrogen. The tissue was then weighed into 200 mg aliquots. Amino acid sequences of the transgenic proteins were used as a reference and “empty vector” control plants were used as a negative control.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Camelina Sativa (false Flax) (myagrum Sativum)

TISSUE(S): Photosynthetic Cell, Leaf

SUBMITTER: Simina Vintila  

LAB HEAD: Manuel Kleiner

PROVIDER: PXD031909 | Pride | 2025-06-30

REPOSITORIES: Pride