Proteomics

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On-tissue spatial proteomics integrating MALDI-MS imaging with shotgun proteomics reveals soy consumption-induced potential candidate markers in a fragile X syndrome mouse model


ABSTRACT: Soy-based diets are associated with increased seizures and autism. Thus, there is an acute need for unbiased protein biomarker identification in Fragile X syndrome (FXS) in response to soy consumption. Herein, we present a spatial proteomics approach integrating mass spectrometry imaging (MSI) with label-free proteomics in a mouse model of FXS to map the spatial distribution and quantify the levels of proteins in the hippocampus and hypothalamus brain regions. In total, 1,250 unique peptides were spatially resolved, demonstrating the diverse array of peptidomes present in the tissue slices and the broad coverage of the strategy. A group of proteins that are known to be involved in glycolysis and synaptic transmission has been identified. The co-expression network analysis indicated that proteins in the soy-based diet group were significantly associated with metabolism and synapse modules in the Fmr1KO brain. Ultimately, this spatial proteomics work is a step toward identifying potential candidate protein markers and novel therapeutic targets for FXS

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Parkinson's Disease,Neuroblastoma

SUBMITTER: Min Ma  

LAB HEAD: Lingjun Li

PROVIDER: PXD034326 | Pride | 2024-02-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
04_HPC_R1.raw Raw
04_HPC_R2.raw Raw
04_HPC_R3.raw Raw
04_HPT_R1.raw Raw
04_HPT_R2.raw Raw
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