Dataset Information

The architecture and pathogenesis of Pseudomonas-Candida biofilms in infections and their influence on the microenvironment

ABSTRACT: Interkingdom Pseudomonas-Candida biofilms is a human pathogen associated with acute and chronic infection. This study examine hoe their structural organization influences their in vivo microenvironment, which in turns affects the interaction of Pseudomonas-Candida biofilms with host immune responses. NCI-H292 grown P. aeruginosa biofilms with and without C. albicans biofilms were used for proteomic analysis. Biofilms were demonstrated in all groups which have 357 proteins from proteomic analysis including i) proteins in PA+CA were higher than PA (76 proteins, such as sigma factor AlgU negative regulatory protein; MucA) and ii) proteins in PA+CA were lower than PA (281 proteins, including alginate genes such as Alginate biosynthesis transcriptional regulatory protein; AlgB). Although most of Pseudomonas proteins in PA+CA were lower than PA due to the lower initial bacteria in preparation of mixed-organism biofilms, mucA overexpressed in PA+CA indicating that high alginate production that transcriptionally controlled by algU-mucABCD genes. These results demonstrated that Pseudomonas-Candida biofilms produced alginate more compared to Pseudomonas biofilms on lungs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Jiradej Makjaroen

LAB HEAD: Asada Leelahavanichkul

PROVIDER: PXD034528 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Rapid Synergistic Biofilm Production of Pseudomonas and Candida on the Pulmonary Cell Surface and in Mice, a Possible Cause of Chronic Mixed Organismal Lung Lesions.

Phuengmaung Pornpimol P Mekjaroen Jiradej J Saisorn Wilasinee W Chatsuwan Tanittha T Somparn Poorichaya P Leelahavanichkul Asada A

International journal of molecular sciences 20220816 16

Due to the possible co-presence of Pseudomonas aeruginosa and Candida albicans (the most common nosocomial pathogens) in lungs, rapid interkingdom biofilm production is possible. As such, PA+CA produced more dominant biofilms on the pulmonary epithelial surface (NCI-H292) (confocal fluorescent extracellular matrix staining) with dominant psl upregulation, as demonstrated by polymerase chain reaction (PCR), after 8 h of experiments than PA alone. With a proteomic analysis, rh ...[more]

PMID: 36012475

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Dataset Information

The architecture and pathogenesis of Pseudomonas-Candida biofilms in infections and their influence on the microenvironment

Publications

Rapid Synergistic Biofilm Production of <i>Pseudomonas</i> and <i>Candida</i> on the Pulmonary Cell Surface and in Mice, a Possible Cause of Chronic Mixed Organismal Lung Lesions.

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