DNA sequence and chromatin modifiers cooperate to confer epigenetic bistability at imprinting control regions

ABSTRACT: DNA methylation is essential for embryonic development and implicated in the regulation of genomic imprinting. Genomic imprinting is established in the germline through parent-specific methylation of distinct cis-regulatory DNA sequences, called imprinting control regions (ICRs). Which factors bind to the opposing chromatin states at ICRs within the same nuclear environment was not systematically addressed. By using a proximity labelling approach with the methylation sensitive transcription factor ZFP57, we identified ATF7IP and other major components of the epigenetic maintenance machinery at ICRs.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Tuncay Baubec  

LAB HEAD: Tuncay Baubec

PROVIDER: PXD034918 | Pride | 2023-03-10

REPOSITORIES: Pride