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Deep and fast label-free Dynamic Organellar Mapping - DDA data

ABSTRACT: The Dynamic Organellar Maps (DOMs) approach combines cell fractionation and shotgun-proteomics for global profiling analysis of protein subcellular localization. Here, we have drastically enhanced the performance of DOMs through data-independent acquisition (DIA) mass spectrometry (MS). DIA-DOMs achieve twice the depth of our previous workflow in the same MS runtime, and substantially improve profiling precision and reproducibility. This repository contains all DDA-LFQ datasets used in our work: A reference dataset acquired in single shot on 100 min gradients and three fractionated datasets providing measured libraries for DIA searches on 100 min, 44 min and 21 min gradients.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Julia Schessner

LAB HEAD: Georg H.H.

PROVIDER: PXD034962 | Pride | 2023-08-25

REPOSITORIES: Pride

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Deep and fast label-free Dynamic Organellar Mapping - DIA datasets

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