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Deep and fast label-free Dynamic Organellar Mapping - starvation + lysosomal pH block

ABSTRACT: The Dynamic Organellar Maps (DOMs) approach combines cell fractionation and shotgun-proteomics for global profiling analysis of protein subcellular localization. Here, we have drastically enhanced the performance of DOMs through data-independent acquisition (DIA) mass spectrometry (MS). DIA-DOMs achieve twice the depth of our previous workflow in the same MS runtime, and substantially improve profiling precision and reproducibility. We then applied DIA-DOMs to capture subcellular localization changes in response to starvation and disruption of lysosomal pH in HeLa cells, which revealed a subset of Golgi proteins that cycle through endosomes. This repository contains the raw data for the comparative experiment.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Julia Schessner

LAB HEAD: Georg H.H. Borner

PROVIDER: PXD034969 | Pride | 2023-08-25

REPOSITORIES: Pride

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			Action	DRS
	20210410_EXPL1_ViAl_SA_HeLa_DIA100min_MQlive_Nt_rep1_12K.raw	Raw
	20210410_EXPL1_ViAl_SA_HeLa_DIA100min_MQlive_Nt_rep1_1K.raw	Raw
	20210410_EXPL1_ViAl_SA_HeLa_DIA100min_MQlive_Nt_rep1_24K.raw	Raw
	20210410_EXPL1_ViAl_SA_HeLa_DIA100min_MQlive_Nt_rep1_3K.raw	Raw
	20210410_EXPL1_ViAl_SA_HeLa_DIA100min_MQlive_Nt_rep1_6K.raw	Raw

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