Transcription profiling of Saccharomyces cerevisiae producing carotenoid cells

ABSTRACT: To obtain insight in the genome-wide response of heterologous carotenoid production in Saccharomyces cerevisiae, we have analyzed the transcriptome of S. cerevisiae strains overexpressing carotenogenic genes from the yeast Xanthophyllomyces dendrorhous. For this purpose, two strains producing different levels of carotenoids were grown in carbon-limited continuous cultures and genome-wide expression was analyzed. The strain producing low carotenoid levels did not exhibit a clear genome-wide transcriptional response, suggesting that low carotenoid levels do not result in cellular stress. Transcriptome analysis of a strain producing high carotenoid levels resulted in specific induction of genes involved in pleiotropic drug resistance (PDR). These genes encode ATP-binding cassette (ABC) type t

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-8451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress