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Mapping diversity in African trypanosomes using high resolution spatial proteomics

ABSTRACT: African trypanosomes are dixenous eukaryotic parasites that impose a significant human and veterinary disease burden on sub-Saharan Africa. Diversity between species and life-cycle stages is concomitant with distinct host and tissue tropisms within this group. Here, the spatial proteomes of two African trypanosome species, Trypanosoma brucei and Trypanosoma congolense, have been mapped, each in mammalian and insect life-stages represented by bloodstream form (BSF) and procyclic form (PCF) respectively. Using the hyperLOPIT (hyperplexed localisation of organelle proteins by isotope tagging) methodology, this work has provided four highly comprehensive spatial proteomes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Trypanosoma Congolense Trypanosoma Brucei

TISSUE(S): Procyclic Form, Bloodstream Form

SUBMITTER: Nicola Moloney

LAB HEAD: Paula MacGregor

PROVIDER: PXD035426 | Pride | 2023-10-22

REPOSITORIES: Pride

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Mapping diversity in African trypanosomes using high resolution spatial proteomics.

Moloney Nicola M NM Barylyuk Konstantin K Tromer Eelco E Crook Oliver M OM Breckels Lisa M LM Lilley Kathryn S KS Waller Ross F RF MacGregor Paula P

Nature communications 20230721 1

African trypanosomes are dixenous eukaryotic parasites that impose a significant human and veterinary disease burden on sub-Saharan Africa. Diversity between species and life-cycle stages is concomitant with distinct host and tissue tropisms within this group. Here, the spatial proteomes of two African trypanosome species, Trypanosoma brucei and Trypanosoma congolense, are mapped across two life-stages. The four resulting datasets provide evidence of expression of approximately 5500 proteins per ...[more]

PMID: 37479728

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