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IER3-AS1 interacting proteins by AP-MS

ABSTRACT: Characterisation IER3-AS1 interacting proteins using chromatin oligo-affinity precipitation (ChOP) followed by mass spectrometry. The HeLa cell lysates was incubated with biotinylated antisense oligonucleotides (ASO), targeting an experimental target antisense long noncoding RNA IER3-AS1 or a control RNA LacZ. LacZ and IER3-AS1 interacting proteomes were pulldown using Streptavidin beads. The eluted protein samples from both LacZ control ASOs and IER3-AS1 ASOs subjected to mass-spectrometry analyses to identify IER3-AS1 interacting proteins.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ekaterina Mirgorodskaya

LAB HEAD: Chandrasekhar Kanduri

PROVIDER: PXD035704 | Pride | 2022-10-15

REPOSITORIES: Pride

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HnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells.

Mahale Sagar S Setia Meenakshi M Prajapati Bharat B Subhash Santhilal S Yadav Mukesh Pratap MP Thankaswamy Kosalai Subazini S Deshpande Ananya A Kuchlyan Jagannath J Di Marco Mirco M Westerlund Fredrik F Wilhelmsson L Marcus LM Kanduri Chandrasekhar C Kanduri Meena M

Nature communications 20220829 1

Although antisense transcription is a widespread event in the mammalian genome, double-stranded RNA (dsRNA) formation between sense and antisense transcripts is very rare and mechanisms that control dsRNA remain unknown. By characterizing the FGF-2 regulated transcriptome in normal and cancer cells, we identified sense and antisense transcripts IER3 and IER3-AS1 that play a critical role in FGF-2 controlled oncogenic pathways. We show that IER3 and IER3-AS1 regulate each other's transcription th ...[more]

PMID: 36038571

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