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Adaptor molecule of DNA methylation in IECs

ABSTRACT: We previously reported that gut microbiota induce de novo DNA methylation of the TLR4 gene in intestinal epithelial cells. Since DNA methyltransferase (DNMT) 3 mediates the transfer of methyl groups to any gene undergoing de novo methylation, the question of how gut microbiota induce the recruitment of DNMT3 to specific genes, including TLR4, remains to be addressed. In this study, we tried to identify an adaptor molecule that recruits DNMT3b to the TLR4 gene by comparing expression of DNMT3-interacting proteins between IEC lines with hypermethylated and hypomethylated TLR4 gene.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Kyoko Takahashi

LAB HEAD: Kyoko Takahashi

PROVIDER: PXD035777 | Pride | 2023-03-11

REPOSITORIES: Pride

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Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions.

Narabayashi Hikari H Koma Chiharu C Nakata Kazuaki K Ikegami Mion M Nakanishi Yusuke Y Ogihara Jun J Tsuda Masato M Hosono Akira A Hanazawa Shigemasa S Takahashi Kyoko K

Frontiers in molecular biosciences 20221021

The intestine is inhabited by a large number of commensal bacteria that are immunologically non-self, potentially causing inflammation. However, in a healthy intestine, inflammation is strictly controlled at low levels to maintain homeostasis. We previously reported that the gut microbiota induce DNA methylation of the gene encoding Toll-like receptor (TLR) 4, a pattern recognition receptor that recognizes lipopolysaccharides of gram-negative bacteria, in colonic epithelial cells, suggesting its ...[more]

PMID: 36339704

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