Proteomics

Dataset Information

0

Molecular mechanisms of endothelial osteoinductive/osteosupressive properties

ABSTRACT: In vivo, osteogenesis is tightly connected with capillaries and endothelium assumed to be one of the main regulators of osteogenic differentiation of underling mesenchymal cell. In our experiments in vitro, we cultured human osteoblasts and human umbilical vein cord endothelial cells (HUVEC) in osteogenic conditions in direct joint culture and in separated joint culture where cells were separated with membrane permeable for proteins, but not for the cells. We revealed that in direct co-cultivation endothelial cells enhance osteoblast differentiation, but in separated co-cultures endothelium completely inhibit osteogenic differentiation. To understand molecular mechanisms of these osteoinductive/osteosupressive properties we performed shotgun proteomics analysis of control cultures and cells from joint cultures. To separate cells from direct co-culture we used magnetic sorting.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell Of Umbilical Vein, Osteoblast

SUBMITTER: Arseniy Lobov  

LAB HEAD: Anna Malashicheva

PROVIDER: PXD037385 | Pride | 2025-05-06

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
10_cd31-_Slot1-27_1_3338.d.zip Other
10_cd31-_Slot1-27_1_3338_uncalibrated.mgf Mgf
10_cd31__Slot1-33_1_3342.d.zip Other
10_cd31__Slot1-33_1_3342_uncalibrated.mgf Mgf
10_huv_c_Slot1-10_1_3321.d.zip Other
Items per page:
1 - 5 of 51
altmetric image

Publications

Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling.

Perepletchikova Daria D   Kuchur Polina P   Basovich Liubov L   Khvorova Irina I   Lobov Arseniy A   Azarkina Kseniia K   Aksenov Nikolay N   Bozhkova Svetlana S   Karelkin Vitaliy V   Malashicheva Anna A  

Cell communication and signaling : CCS 20250219 1

<h4>Background</h4>Angiogenesis and osteogenesis are closely interrelated. The interaction between endothelial and bone-forming cells, such as osteoblasts, is crucial for normal bone development and repair. Juxtacrine and paracrine mechanisms play key roles in cell differentiation towards the osteogenic direction, assuming the direct effect of endothelium on osteogenic differentiation. However, the mechanisms of this interplay have yet to be thoroughly studied.<h4>Methods</h4>Isolated endothelia  ...[more]

Similar Datasets

Proteomics Comparative analysis of DDA- and DIA-PASEF methods for proteomics analysis of human valve interstitial cells and osteoblasts during osteogenic differentiation
2024-11-12 | PXD036566 | Pride
Transcriptomics hOB Transcriptome after HUVEC-Cocultivation
2015-12-17 | E-MTAB-4152 | biostudies-arrayexpress
Proteomics Comparative analysis of proteomes and secretomes of human mesenchymal stem cells isolated from six sources during osteogenic differentiation
2023-11-05 | PXD037320 | Pride
Transcriptomics Transcription profiling of human bone marrow mesenchymal stromal cells before and after stimulation with dexamethasone to study the early molecular processes of osteogenic differentiation
2009-09-18 | E-GEOD-18043 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human melanoma-endothelial cells co-cultured vs controls to investigate cellular communication
2008-10-18 | E-GEOD-8699 | biostudies-arrayexpress
Transcriptomics Modeling Familial Cancer with iPSC Approaches
2015-04-10 | E-GEOD-58123 | biostudies-arrayexpress
Proteomics Crenigacestat inhibits osteogenic differentiation of human valve interstitial cells and seems promising for treatment of cardiovascular calcification
2022-10-04 | PXD029632 | Pride
Transcriptomics Gene expression profiles of dental follicle cells after 7 days of differentiation in vitro with BMP2, IGF2 and dexamethasone
2010-11-10 | E-GEOD-20963 | biostudies-arrayexpress
Proteomics Long non-coding RNA CASC2 regulates osteoblasts matrix mineralization
2023-06-14 | PXD040463 | Pride
Proteomics Conditioned medium from human iPSC-derived mesenchymal progenitors: Effect of extended progenitor cultivation vs. osteogenic pre-differentiation on the medium stimulatory activity
2025-05-07 | PXD052766 | Pride