Dataset Information

Cellular quiescence uncouples the proteome from the transcriptome

ABSTRACT: Neural stem cells (NSCs) are multipotent cells in the central nervous system which can self-renew, differentiate or reversibly exit the cell cycle to enter a dormat state known as quiescence. To study the molecular mechanisms underpinning this state we use an in vitro model of NSC quiescence, which uses adult hippocampal NSCs harvested from mice. In this cell culture system, we are able to reversibly induce quiescence by supplementing the media with BMP4. In this study we examine how the proteome changes as NSCs transition from an active state (proliferating, 0d BMP4) into quiescence (up to 21 days in BMP4).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Stem Cell

SUBMITTER: Mark Skehel

LAB HEAD: Francois Guillemot

PROVIDER: PXD037487 | Pride | 2026-03-09

REPOSITORIES: Pride

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Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.

Rossi Alice A Coum Antoine A Madelenat Manon M Harris Lachlan L Strohbuecker Stephanie S Chai Andrea A Fiaz Hania H Chaouni Rita R Faull Peter P Costello Heaven Neve N Grey William W Bonnet Dominique D Hamid Fursham F Makeyev Eugene V EV Snijders Ambrosius P AP Kelly Gavin G Guillemot François F Sousa-Nunes Rita R

The EMBO journal 20260205

Quiescence is a cellular state defined by reversible cell-cycle arrest and diminished biosynthesis, particularly of nucleic acids and proteins. These features protect stem cells from proliferation-induced mutations, self-renewal exhaustion, and environmental insults. Despite relevance to development, tissue homeostasis and cancer, we lack understanding about many aspects of quiescence regulation and unique molecular markers for this state. Here, we employ Drosophila and mammalian neural stem cel ...[more]

PMID: 41645004

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