Proteomics

Dataset Information

0

Peroxynitrite-mediated post-translational modification of human glutamine synthetase

ABSTRACT: The aim of the project was to understand the mechanisms that underlie the oxidative inactivation of human glutamine synthetase (HsGS). For that purpose, we expressed and purified recombinant HsGS, exposed it in phosphate buffer to peroxynitrite (ONOO-) and assessed the functional and structural consequences. To characterize the oxidative post-translational modifications induced by ONOO-, several approaches were carried out (e.g. SDS-PAGE, western-blot, amino acid quantification by LC, etc). The most detailed characterization was performed through LC-MS/MS analysis after trypsin digestion of the ONOO--treated protein, which allowed us to identify the amino acid residues that are modified and to estimate the extent of such modifications.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nicolás Campolo  

LAB HEAD: Rafael Radi

PROVIDER: PXD037583 | Pride | 2023-05-10

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Multiple oxidative post-translational modifications of human glutamine synthetase mediate peroxynitrite-dependent enzyme inactivation and aggregation.

Campolo Nicolás N   Mastrogiovanni Mauricio M   Mariotti Michele M   Issoglio Federico M FM   Estrin Darío D   Hägglund Per P   Grune Tilman T   Davies Michael J MJ   Bartesaghi Silvina S   Radi Rafael R  

The Journal of biological chemistry 20230123 3

Glutamine synthetase (GS), which catalyzes the ATP-dependent synthesis of L-glutamine from L-glutamate and ammonia, is a ubiquitous and conserved enzyme that plays a pivotal role in nitrogen metabolism across all life domains. In vertebrates, GS is highly expressed in astrocytes, where its activity sustains the glutamate-glutamine cycle at glutamatergic synapses and is thus essential for maintaining brain homeostasis. In fact, decreased GS levels or activity have been associated with neurodegene  ...[more]

Similar Datasets

Proteomics Cross-linking/mass spectrometry for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
2019-10-07 | PXD010884 | Pride
Transcriptomics RNA-seq experiment to investigate the effect of inactivating the Apoptosis Antagonizing Transcription Factor (Aatf)
2019-03-31 | E-MTAB-7077 | biostudies-arrayexpress
Transcriptomics RNA-seq of iron-rich pelagic aggregates (iron snow) in 2017 and 2018
2021-10-15 | E-MTAB-9686 | biostudies-arrayexpress
Proteomics High-density chemical cross-linking for modeling protein interactions
2019-12-06 | PXD011296 | Pride
Transcriptomics MethylationEPIC array from fresh oral and oropharyngeal tissue samples from head and neck squamous cell carcinoma patients, tissue samples from potentially premalignant oral lesions and fresh tonsil tissue samples from control patients
2024-04-22 | E-MTAB-13954 | biostudies-arrayexpress
Transcriptomics RNA-seq to investigate gene expression profile associated with long-non coding RNA RAIN in TPC1 cells
2018-09-01 | E-MTAB-6622 | biostudies-arrayexpress
Proteomics Transformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1
2018-07-13 | PXD010088 | Pride
Genomics Human variation in PolII and NF-KappaB binding (ChIP-seq study with NF-KappaB)
2010-03-22 | E-GEOD-19485 | biostudies-arrayexpress
Proteomics Identification of Siglec-7-ligand candidates on JVM-3 human B cell line by proximity labeling
2022-05-31 | PXD024690 | Pride
Proteomics A Triple KnockOut Isobaric-labelling Quality Control Platform in an Integrated On-line Database Search
2020-03-31 | PXD017803 | Pride