Proteomics

Dataset Information

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Mouse osteoclast secretory lysosomes nLC-ESI-MS/MS - WT vs Slc37a2 KO


ABSTRACT: To survey the proteome of osteoclast secretory lysosomes, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed’ with SPIONs to encourage uptake into endosomes and then ‘chased’ into secretory lysosomes upon the convergence of SPION-loaded endosomes with lysosomes and secretory pathways. Following the ‘pulse-chase’, osteoclasts were homogenized, SPION-loaded organelles captured-from post-nuclear supernatants using magnetic columns, and enriched organelles eluted and processed for 1D in-gel digestion and mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bone Marrow, Macrophage

SUBMITTER: Amy Ribet  

LAB HEAD: Nathan Pavlos

PROVIDER: PXD037813 | Pride | 2023-01-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
3K1.raw Raw
3K10.raw Raw
3K2.raw Raw
3K3.raw Raw
3K4.raw Raw
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