Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Beta Defensin-2-mediated protection of human skin barrier in vitro; a mechanistic evaluation

ABSTRACT: I this project we performed global proteomic and secretomic analyses, following HBD2 stimulation, with or without V8-mediated damage, demonstrating minimal impact of the peptide alone on undamaged cells and showing that HBD2 did not induce expression of known antiproteases, but rather may function by modulating expression of extracellular matrix proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Skin

DISEASE(S): Atopic Dermatitis

SUBMITTER: Jimi Wills

LAB HEAD: Donald J. Davidson

PROVIDER: PXD037844 | Pride | 2023-02-09

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	cells02_hBD2-V8-1.raw	Raw
	cells03_TNFa-1.raw	Raw
	cells04_V8-1.raw	Raw
	cells05_WT-1.raw	Raw
	cells06_IL1B100-1.raw	Raw

Items per page:

1 - 5 of 73

Similar Datasets

Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression

Project description:Kidney fibrosis, characterized by excessive extracellular matrix (ECM) deposition, is a progressive disease that, despite affecting 10% of the population, lacks specific treatments and suitable biomarkers. Aimed at unraveling disease mechanisms and identifying potential therapeutic targets, this study presents a comprehensive, time-resolved multi-omics analysis of kidney fibrosis using an in vitro model system based on human kidney PDGFRÎ²+ mesenchymal cells. Using computational network modeling we integrated transcriptomics, proteomics, phosphoproteomics, and secretomics with imaging of the extracellular matrix (ECM). We quantified over 14,000 biomolecules across seven time points following TGF-Î² stimulation, revealing distinct temporal patterns in the expression and activity of known and potential novel renal fibrosis markers and modulators. The resulting time-resolved multi-omic network models allowed us to propose mechanisms related to fibrosis progression through early transcriptional reprogramming. Using siRNA knockdowns and phenotypic assays, we validated predictions and elucidated regulatory mechanisms underlying kidney fibrosis. Notably, we demonstrate that several early-activated transcription factors, including FLI1 and E2F1, act as negative regulators of collagen deposition and propose underlying molecular mechanisms. This work advances our understanding of the pathogenesis of kidney fibrosis and provides a valuable resource for the organ fibrosis research community.

2025-04-08 | PXD056096 | Pride

Genetic factors that respond to N in maize leaf tissue

Project description:Genotype and nitrogen-dosage effect on maize leaves collected at V8 leaf stage B73 is a model maize genotype while Illinois high protein line (IHP) is a metabolic extreme selected for higher grain protein concentration. It is a well known fact that the leaves serve as source and earshoot as a sink. Microarray analysis of V8 leaf collected from B73 and IHP genotypes grown at vairable nitrogen applications. Keywords: Genotype and N-treatment response

2009-12-11 | GSE18011 | GEO

A mechanistic evaluation of human beta defensin 2 mediated protection of human skin barrier in vitro.

Project description:The human skin barrier, a biological imperative, is impaired in inflammatory skin diseases such as atopic dermatitis (AD). Staphylococcus aureus is associated with AD lesions and contributes to pathological inflammation and further barrier impairment. S. aureus secretes extracellular proteases, such as V8 (or 'SspA'), which cleave extracellular proteins to reduce skin barrier. Previous studies demonstrated that the host defence peptide human beta-defensin 2 (HBD2) prevented V8-mediated damage. Here, the mechanism of HBD2-mediated barrier protection in vitro is examined. Application of exogenous HBD2 provided protection against V8, irrespective of timeline of application or native peptide folding, raising the prospect of simple peptide analogues as therapeutics. HBD2 treatment, in context of V8-mediated damage, modulated the proteomic/secretomic profiles of HaCaT cells, altering levels of specific extracellular matrix proteins, potentially recovering V8 damage. However, HBD2 alone did not substantially modulate cellular proteomic/secretomics profiles in the absence of damage, suggesting possible therapeutic targeting of lesion damage sites only. HBD2 did not show any direct protease inhibition or induce expression of known antiproteases, did not alter keratinocyte migration or proliferation, or form protective nanonet structures. These data validate the barrier-protective properties of HBD2 in vitro and establish key protein datasets for further targeted mechanistic analyses.

| S-EPMC9908873 | biostudies-literature

Proteomics analysis of whole-cell, soma and neuronal projection fractions from day 35 iNeurons in Control and ASAH1-/-

Project description:This experiment describes the quantitative proteomic profiling of whole-cell, soma, and neuronal projection fractions from day 35 induced neurons (iN) derived from control and ASAH1 knockout human embryonic stem cells. Soma and projection compartments were physically separated using Transwell culture inserts, and triplicate biological replicates were processed using TMTpro 18-plex labeling and high-resolution Orbitrap mass spectrometry with FAIMS. Data were searched against the human proteome with stringent FDR control and reporter ion quantification, enabling compartment-resolved analysis of proteome alterations associated with ASAH1 deficiency.

2025-08-12 | PXD067229 | Pride

Comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulators for cellulase and xylanase gene expression

Project description:In this study,comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106 were employed to screen for novel regulators for cellulase and xylanase gene expression.

2016-08-31 | GSE80076 | GEO

Pungitius pungitius isolate:FIN-PYO-11F

Project description:Pungitius pungitius v8 assembly

| PRJNA1045347 | ENA

Quantitative LC-MS/MS analysis of secretome of Mesenchymal Cells from Tooth Germ of Mice

Project description:Label free: The aim of project is to identify the proteins that may have a role in the inductive signal of mesenchymal cells. Protein identification and quantitively compare the proteins from the following samples: 1- Exosomes purified from embryonic mesenchymal cells of tooth germ after overnight culture in 2D and 3D conditions 2- Secretome of embryonic mesenchymal cells of tooth germ after overnight 3D culture. 3- Embryonic mesenchymal cells from tooth germ- fresh frozen as pellet TMT Samples 10.5 and 14.5 are exosomes derived from immortalized cell line and different stages of tooth embryonic development. These sets of samples were seeded in well plate. The embryonic samples are a pool of 6 biological replicates. The aim is to identify the different proteins between these stages as well as the cell line.

2025-05-07 | PXD053767 | Pride

Genetic factors that respond to N in maize leaf tissue

2010-05-18 | E-GEOD-18011 | biostudies-arrayexpress

Aspergillus flavus gene expression profile under different conditions of ustiloxin production

Project description:Transcriptome data of A. flavus was assembled under four different conditions (two strains and two media) for omics data analyses of genes related to ustiloxin production. The used two strains were the over-expressed strain (ustR-oe) of the transcription factor in ustiloxin gene cluster, ustR, and the pyrG marker complemented strain (control). The two media were V8 juice liquid and potato dextrose broth (PDB) media. The ustR-oe strain produced more ustiloxin B than the control strain, and the production is much higher in V8 than in PDB. Therefore, four different state of transcriptome could be obtained under these four conditions. The obtained transcriptome data was combined with the GEO data acc. no. GSE15435 and uesd for omics data analyses.

2020-04-17 | GSE136041 | GEO

Transcription profiling of Arabidopsis plants challenged with Pieris rapae larvae for 3-5 h and then left unchallenged for 24 hr

Project description:Arabidopsis plants were challenged with Pieris rapae larvae for 3-5 h and then left unchallenged for 24 hr. Local (damaged) and distal (undamaged) leaves were harvested

2004-11-01 | E-MEXP-163 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data