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Ureter proteomics analysis following stent placement and removal in a porcine model

ABSTRACT: Symptoms including pain and discomfort commonly present during ureteral stenting is not well understood. We investigated global changes in ureters at the transcriptional, translational, and functional levels while stents are indwelling and following removal and recovery using a porcine model. Polaris stents (6Fr, Boston Scientific, Natick, MA) were cystoscopically inserted with placement confirmed using fluoroscopy. For proteomics samples, ureters were stented for 14 days and recovered for 7 days and each pig was stented unilaterally so that the contralateral unstented kidney and ureter served as an internal control.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Ureter Urothelial Cell, Ureter

DISEASE(S): Wounds And Injuries

SUBMITTER: Hans Adomat

LAB HEAD: Dirk Lange

PROVIDER: PXD038161 | Pride | 2023-05-10

REPOSITORIES: Pride

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Indwelling stents cause severe inflammation and fibrosis of the ureter via urothelial-mesenchymal transition.

Reicherz Alina A Eltit Felipe F Scotland Kymora K Almutairi Khaled K Bell Robert R Mojtahedzadeh Bita B Cox Michael M Chew Ben B Lange Dirk D

Scientific reports 20230404 1

To explore the pathways and mechanisms driving inflammation and fibrosis in stented ureters. In total, six healthy female pigs underwent cystoscopic unilateral ureteral stent insertion (6 Fr). After 14 days indwelling time, ureteral tissue was harvested in three pigs, while the remaining three pigs had their stents removed, and were recovered for 7 days. Three separate pigs served as controls. Tissue from stented and contralateral ureters was analysed histologically to evaluate tissue remodellin ...[more]

PMID: 37015949

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