Proteomics

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Interactions of graphene-based materials with the blood-brain barrier

ABSTRACT: In this work, we performed an in vitro investigation of GRM interactions with the BBB. To this aim,we employed GRMs with different surface chemistry and stability, both murine and human models of increasing complexity, and a portfolio of complementary label-free analytical techniques. The use of label-free strategies allows to avoid the problem of fluorescent tag interference and leaching, which makes the results on the bionano-interactions difficult to interpret.42-43 The workflow developed in this paper is widely applicable to study the translocation of different micro- and nano-materials and represents a valuable method to reduce the more expensive and ethically concerning in vivo biodistribution studies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Andrea Armirotti  

LAB HEAD: Andrea Armirotti

PROVIDER: PXD038297 | Pride | 2023-05-10

REPOSITORIES: Pride

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Thanks to their biocompatibility and high cargo capability, graphene-based materials (GRMs) might represent an ideal brain delivery system. The capability of GRMs to reach the brain has mainly been investigated <i>in vivo</i> and has highlighted some controversy. Herein, we employed two <i>in vitro</i> BBB models of increasing complexity to investigate the bionano interactions with graphene oxide (GO) and few-layer graphene (FLG): a 2D murine Transwell model, followed by a 3D human multicellular  ...[more]

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