Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Cell Culture
SUBMITTER: Romina Belli
LAB HEAD: Peter Arthur Irene De Wulf
PROVIDER: PXD038337 | Pride | 2023-07-20
REPOSITORIES: Pride
Smurova Ksenia K Damizia Michela M Irene Carmela C Stancari Stefania S Berto Giovanna G Perticari Giulia G Iacovella Maria Giuseppina MG D'Ambrosio Ilaria I Giubettini Maria M Philippe Réginald R Baggio Chiara C Callegaro Elisabetta E Casagranda Andrea A Corsini Alessandro A Polese Vincenzo Gentile VG Ricci Anna A Dassi Erik E De Wulf Peter P
Nature communications 20230601 1
Kinetochores assemble on centromeres via histone H3 variant CENP-A and low levels of centromere transcripts (cenRNAs). The latter are ensured by the downregulation of RNA polymerase II (RNAPII) activity, and cenRNA turnover by the nuclear exosome. Using S. cerevisiae, we now add protein kinase Rio1 to this scheme. Yeast cenRNAs are produced either as short (median lengths of 231 nt) or long (4458 nt) transcripts, in a 1:1 ratio. Rio1 limits their production by reducing RNAPII accessibility and p ...[more]