Dataset Information

Proteomic analysis of Panax ginseng leaves subjected to salinity stress

ABSTRACT: Ginseng is an important crop in East Asia due to its medicinal and nutritional benefits originating from ingredients such as the ginsenosides. Numerous researches have been directed to cultivate ginseng with high yield especially targeting its growth and development for protection against abiotic stresses, which are affecting both the yield and quality. Particularly, salinity has been characterized as a major abiotic stressor that affects the annual yield of ginseng. Therefore, to characterize the salt-responsive proteins in the ginseng plant, ginseng leaves were harvested post-treatment with salt in a time-dependent manner. Utilizing a label-free quantitative proteome analysis approach, this study identified a total of 2,484 proteins. Among them, 468 proteins showed a significant modulation in their abundance among ginseng leaf samples at 4 different time points (0, 24, 72, 96 h) following salt stress. Further functional classification revealed that catalase-peroxidase 2, voltage-gated potassium channel subunit beta-2, fructose-1,6-bisphosphatase class 1, and chlorophyll a-b binding protein associated proteins accumulated in response to the salt stress. Of these, glycosyl hydrolase 17 (GH17) showed similar abundance profiles at both the transcript and proteome level. Therefore, for further understanding of GH17 underlying salt stress response mechanism, GH17 overexpressing transgenic Arabidopsis were generated. In response to salt stress, transgenic plants uncovered a tolerance phenotype without compromising plant growth. The proteome alterations in response to salt stress presented here resulted in identifying a protein GH17 with a potential key role in salt-stressed ginseng.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Panax Ginseng

TISSUE(S): Leaf

SUBMITTER: Sun Tae Kim

LAB HEAD: Sun Tae Kim

PROVIDER: PXD038524 | Pride | 2023-03-11

REPOSITORIES: Pride

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Publications

Proteomic Analysis Reveals a Critical Role of the Glycosyl Hydrolase 17 Protein in <i>Panax ginseng</i> Leaves under Salt Stress.

Jung Ju-Young JY Min Cheol Woo CW Jang Jeong Woo JW Gupta Ravi R Kim Ji-Hyun JH Kim Young-Hun YH Cho Sung Won SW Song Young Hun YH Jo Ick-Hyun IH Rakwal Randeep R Kim Yu-Jin YJ Kim Sun Tae ST

International journal of molecular sciences 20230212 4

Ginseng, an important crop in East Asia, exhibits multiple medicinal and nutritional benefits because of the presence of ginsenosides. On the other hand, the ginseng yield is severely affected by abiotic stressors, particularly salinity, which reduces yield and quality. Therefore, efforts are needed to improve the ginseng yield during salinity stress, but salinity stress-induced changes in ginseng are poorly understood, particularly at the proteome-wide level. In this study, we report the compar ...[more]

PMID: 36835103

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Project description:Engineered abiotic stress resistance is an important target for increasing agricultural productivity.There are concerns, however, regarding possible ecological impacts of transgenic crops. In contrast to the first wave of transgenic crops, many abiotic stress resistance genes can initiate complex downstream changes. Transcriptome profiling has been suggested as a comprehensive non-targeted approach to examine secondary effects. We compared phenotypic and transcriptomic effects of constitutive expression of genes intended to confer salt stress tolerance by three different mechanisms: a transcription factor, CBF3/DREB1a; a metabolic gene, M6PR, for mannitol biosynthesis; and the Na+/H+ antiporter, SOS1. Transgenic CBF3, M6PR, and SOS1 Arabidopsis thaliana were grown together in the growth chamber, greenhouse and field. In the absence of salt, M6PR and SOS1 lines performed comparably to wild type; CBF3 lines exhibited dwarfing as reported previously. All three transgenes conferred fitness advantage when subjected to 100 mM NaCl in the growth chamber. CBF3 and M6PR affected transcription of numerous abiotic stress- related genes as measured by Affymetrix microarray analysis. M6PR additionally modified expression of biotic- and oxidative- stress genes. Transcriptional effects of SOS1 in the absence of salt were smaller and primarily limited to redox-related genes. The extent of transcriptome change, however, did not correlate with effects on growth and reproduction. Thus, magnitude of global transcriptome differences may not predict phenotypic differences upon which environment and selection act to influence fitness. These observations have implications for interpretation of transcriptome analyses in the context of risk assessment and emphasize importance of evaluation within a phenotypic context. Both transgenic plants and relative WT plants were grown in the growth chamber in the absence and presence of salt stress. Plants from 20 days after sowing (6 days after salt treatment) were used for RNA extraction and hybridization on Affymetrix microarrays. There were two biological replicates for each genotype and salt treatment combination.

Dataset Information

Proteomic analysis of Panax ginseng leaves subjected to salinity stress

Publications

Proteomic Analysis Reveals a Critical Role of the Glycosyl Hydrolase 17 Protein in <i>Panax ginseng</i> Leaves under Salt Stress.

Similar Datasets

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