Ontology highlight
ABSTRACT:
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Cell Suspension Culture
SUBMITTER: Ulrich Stelzl
LAB HEAD: Ulrich Stelzl
PROVIDER: PXD038551 | Pride | 2023-07-20
REPOSITORIES: Pride
Journal of proteome research 20230413 6
BCR-ABL is the oncogenic fusion product of tyrosine kinase ABL1 and a highly frequent driver of acute lymphocytic leukemia (ALL) and chronic myeloid leukemia (CML). The kinase activity of BCR-ABL is strongly elevated; however, changes of substrate specificity in comparison to wild-type ABL1 kinase are less well characterized. Here, we heterologously expressed full-length BCR-ABL kinases in yeast. We exploited the proteome of living yeast as an <i>in vivo</i> phospho-tyrosine substrate for assayi ...[more]