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Type I interferon/STAT1 signaling negative feedback regulates UBE2M-mediated antiviral innate immunity

ABSTRACT: To identify potential proteins involved in RIG-I regulation, we pulled down RIG-I from HEK293T or NIH-3T3 cells overexpressing RIG-I, and the RIG-I-bound proteins were then analyzed by mass spectrometry (MS).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Cell Culture, Fibroblast

DISEASE(S): Influenza

SUBMITTER: Xianghui Kong

LAB HEAD: Zhijian Cai

PROVIDER: PXD039071 | Pride | 2023-01-30

REPOSITORIES: Pride

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			Action	DRS
	IP_RIGI_in_HEK293T.raw	Raw
	IP_RIGI_in_HEK293T.xlsx	Xlsx
	IP_RIGI_in_NIH_3T3.raw	Raw
	IP_RIGI_in_NIH_3T3.xlsx	Xlsx
	IP_UBE2M_in_NIH_3T3.raw	Raw

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Publications

Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner.

Kong Xianghui X Lu Xinliang X Wang Shibo S Hao Jiayue J Guo Danfeng D Wu Hao H Jiang Yu Y Sun Yi Y Wang Jianli J Zhang Gensheng G Cai Zhijian Z

Cell reports 20230119 1

Type I interferon (IFN-I) signaling is central to inducing antiviral innate immunity. However, the mechanisms for IFN-I signaling self-regulation are still largely unknown. Here, we report that RNA virus-infected macrophages with UBE2M deficiency produced decreased IFN-I expression in a RIG-I-dependent manner, causing an aggravated viral infection. Mechanistically, UBE2M inhibits RIG-I degradation by preventing the interaction of RIG-I and E3 ligase STUB1, resulting in antiviral IFN-I signaling ...[more]

PMID: 36662617

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