Dataset Information

Anti-Pol II pS5 co-immunoprecipitation in DIDO WT, KO and ΔSPOC HEK293T cells

ABSTRACT: The analysis of differential interactome of Pol II pS5 in DIDO WT, KO and ΔSPOC HEK293T cells using Pol II pS5 (3E8) antibody coupled to Protein G beads (no crosslinking).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: WeiQiang Chen

LAB HEAD: Dea Slade

PROVIDER: PXD039567 | Pride | 2023-10-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20220524_E3_RSLC1_Benedum_Slade_MPL_UniWien_pSer5_DIDO_KO1_s13_40p.raw	Raw
	20220524_E3_RSLC1_Benedum_Slade_MPL_UniWien_pSer5_DIDO_KO2_s14_40p.raw	Raw
	20220524_E3_RSLC1_Benedum_Slade_MPL_UniWien_pSer5_DIDO_KO3_s15_40p.raw	Raw
	20220524_E3_RSLC1_Benedum_Slade_MPL_UniWien_pSer5_DIDO_WT1_s10_40p.raw	Raw
	20220524_E3_RSLC1_Benedum_Slade_MPL_UniWien_pSer5_DIDO_WT2_s11_40p.raw	Raw

Items per page:

1 - 5 of 13

Similar Datasets

Project description:Cellular responses to signalling pathways are often highly dynamic, however most analyses of developmental signalling pathways focus on a single endpoint. We have analyzed the temporal changes in transcription following a short Notch activation treatment and related these to the recruitment of the Notch pathway transcription factor, CSL [Suppressor of Hairless, Su(H), in Drosophila], and to the state of RNA Polymerase II (Pol II) binding. A total of 154 genes showed significant differential expression over time and their expression profiles stratified into 14 clusters based on temporal and quantitative differences in their responses. These differences were partially reflected in the profiles of Pol II and Su(H) binding. However, neither could fully account for the different response profiles. Furthermore, the timing of the different responses was unaffected by more prolonged Notch activation. Instead our data suggest that regulatory relationships between genes that segregate into different response clusters can partially account for the stratification. Thus, feed-forward repression, where products of early responding Enhancer of split bHLH genes (E(spl)bHLH) inhibit expression of endogenous repressors, is one mechanism that explains the profile of genes that exhibit delayed up-regulation. E(spl)bHLH genes may therefore be responsible for co-ordinating the Notch response of a wide spectrum of other targets, explaining their critical functions in many developmental and disease contexts. DmD8 cells were collected at 7 time points (0M-bM-^@M-^Y, 10M-bM-^@M-^Y, 20M-bM-^@M-^Y, 30M-bM-^@M-^Y, 40M-bM-^@M-^Y, 60M-bM-^@M-^Y and 100M-bM-^@M-^Y) after a 5 minute Notch stimulation as 3 independent replicates. Immunoprecipitation was performed with Su(H) antibody and compared to the total input DNA. Samples from replicates #1 and #2 were labelled with Cy5, and replicate #3 was labelled with Cy3 as a dye-swap. For time point 10M-bM-^@M-^Y, only 2 biological replicates were obtained.

Dataset Information

Anti-Pol II pS5 co-immunoprecipitation in DIDO WT, KO and ΔSPOC HEK293T cells

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets