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Directed Differentiation of Human iPSCs into Mesenchymal Lineages by Optogenetic Control of TGF-β Signaling

ABSTRACT: For chondrogenic studies of optogenetically activated TGF-β signaling, optogenetic human iPSC-derived MSCs were encapsulated in hydrogels (20 million cells/mL of 2% agarose hydrogel). Groups received either no soluble TGF-β or optogenetic stimulation, or soluble TGF-β3 alone, or optogenetic stimulation alone. After 21 days of differentiation, we performed global quantitative proteomics on samples from two independent experiments, with n=3 replicates per group.

INSTRUMENT(S): ultraflex

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Josephine Wu

LAB HEAD: Gordana Vunjak-Novakovic

PROVIDER: PXD040232 | Pride | 2023-07-20

REPOSITORIES: Pride

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Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-β signaling.

Wu Josephine Y JY Yeager Keith K Tavakol Daniel Naveed DN Morsink Margaretha M Wang Bryan B Soni Rajesh Kumar RK Hung Clark T CT Vunjak-Novakovic Gordana G

Cell reports 20230512 5

In tissue development and homeostasis, transforming growth factor (TGF)-β signaling is finely coordinated by latent forms and matrix sequestration. Optogenetics can offer precise and dynamic control of cell signaling. We report the development of an optogenetic human induced pluripotent stem cell system for TGF-β signaling and demonstrate its utility in directing differentiation into the smooth muscle, tenogenic, and chondrogenic lineages. Light-activated TGF-β signaling resulted in expression o ...[more]

PMID: 37178118

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