Directed Differentiation of Human iPSCs into Mesenchymal Lineages by Optogenetic Control of TGF-β Signaling

ABSTRACT: For chondrogenic studies of optogenetically activated TGF-β signaling, optogenetic human iPSC-derived MSCs were encapsulated in hydrogels (20 million cells/mL of 2% agarose hydrogel). Groups received either no soluble TGF-β or optogenetic stimulation, or soluble TGF-β3 alone, or optogenetic stimulation alone. After 21 days of differentiation, we performed global quantitative proteomics on samples from two independent experiments, with n=3 replicates per group.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Josephine Wu  

LAB HEAD: Gordana Vunjak-Novakovic

PROVIDER: PXD040232 | Pride | 2023-07-20

REPOSITORIES: Pride