Dataset Information

Immunometabolism in resolving atherosclerosis guides the development of nanometabolite therapy

ABSTRACT: A 6-month in vivo study will be carried out at Case Western Reserve University (CWRU) using an ApoE-/- mouse model of atherosclerosis (obtained from The Jackson Laboratory), which will be treated as follows: Control: Mice fed a chow diet (control) (n=12) Progression: Mice fed a high-fat diet with cholesterol (n=12) Regression: Mice fed a high-fat diet with cholesterol followed by a switch to a chow diet (n=12) At the end of the experiment, mice will be euthanized and aorta excised from each animal. They will be immediately incubated in dialyzed serum-containing medium with uniformly C13 labelled glucose for 24 h and then extracted for Stable Isotope Resolved Metabolomics (SIRM) experiments. The incubation media and tissue extracts will be sent to University of Kentucky’s RC-SIRM: Resource Center for Stable Isotope-Resolved Metabolomics for analysis. The samples will undergo the following modes of analysis: nuclear magnetic resonance (NMR), Ion Chromatography Coupled with Mass Spectrometry (ICMS)

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Aorta

SUBMITTER: Andrei Maiseyeu

LAB HEAD: Andrei Maiseyeu

PROVIDER: PXD041137 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	01_AO4_12CGlc_Ctl_171016_CWRU_AM_slice_ICMSA.raw	Raw
	02_AO1_13C6Glc_Ctl_171016_CWRU_AM_slice_ICMSA.raw	Raw
	03_AO2_13C6Glc_Ctl_171016_CWRU_AM_slice_ICMSA.raw	Raw
	04_AO3_13C6Glc_Ctl_171016_CWRU_AM_slice_ICMSA.raw	Raw
	05_AO5_13C6Glc_Ctl_171016_CWRU_AM_slice_ICMSA.raw	Raw

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Publications

Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution.

Hong Natalie E NE Chaplin Alice A Di Lin L Ravodina Anastasia A Bevan Graham H GH Gao Huiyun H Asase Courteney C Gangwar Roopesh Singh RS Cameron Mark J MJ Mignery Matthew M Cherepanova Olga O Finn Aloke V AV Nayak Lalitha L Pieper Andrew A AA Maiseyeu Andrei A

Cell reports 20241028 11

Current pharmacologic treatments for atherosclerosis do not completely protect patients; additional protection can be achieved by dietary modifications, such as a low-cholesterol/low-fat diet (LCLFD), that mediate plaque stabilization and inflammation reduction. However, this lifestyle modification can be challenging for patients. Unfortunately, incomplete understanding of the underlying mechanisms has thwarted efforts to mimic the protective effects of a LCLFD. Here, we report that the tricarbo ...[more]

PMID: 39466775

Dataset Information

Immunometabolism in resolving atherosclerosis guides the development of nanometabolite therapy

Dataset's files

Publications

Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution.

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