Novel integrated computational AMP discovery approaches highlight diversity in the helminth AMP repertoire

ABSTRACT: Invertebrate antimicrobial peptides (AMPs) form the first line of defence against pathogenic microbes. Understanding AMP profiles in helminths, their importance to helminth biology, and how they shape microbial communities could reveal novel approaches for anthelmintic and/or antimicrobial development. In this study, we employed a LC-MS/MS peptidomics pipeline to validate a novel integrated homology- and computational-based pipeline for the discovery of helminth AMPs. Our peptidomics pipeline found 60 high-confidence PSMs correlating to 15 high-confidence novel AMP-LPs within Ascaris suum body-cavity fluid supporting the need for further characterisation of these peptides and their functions within helminths.

INSTRUMENT(S):

ORGANISM(S): Ascaris Suum

SUBMITTER: Darrin McKenzie  

LAB HEAD: Prof Angela Mousley

PROVIDER: PXD042293 | Pride | 2023-07-26

REPOSITORIES: Pride