Dataset Information

Proteomic Profiling of PPIX-binding proteins between cell types

ABSTRACT: To use a probe connecting PPIX with biotin to affinity purify interacting proteins in various cell lines. Then to use TMT labeling of the purified proteins, as well as those from controls, to construct data sets of proteins identified with mass spec, together with biostatistical analysis, to distinguish valid interactions for further study. These data may cumulatively allow us to delineate trafficking, signaling, or metabolic pathways related to PPIX binding.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Fibroblast

SUBMITTER: Zuo-Fei Yuan

LAB HEAD: John Schuetz

PROVIDER: PXD042631 | Pride | 2023-07-20

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Jschugrp_082318_ppixtmt_fxn_01.1.pepXML	Pepxml
	Jschugrp_082318_ppixtmt_fxn_01.raw	Raw
	Jschugrp_082318_ppixtmt_fxn_02.1.pepXML	Pepxml
	Jschugrp_082318_ppixtmt_fxn_02.raw	Raw
	Jschugrp_082318_ppixtmt_fxn_03.1.pepXML	Pepxml

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Publications

A PPIX-binding probe facilitates discovery of PPIX-induced cell death modulation by peroxiredoxin.

Lynch John J Wang Yao Y Li Yuxin Y Kavdia Kanisha K Fukuda Yu Y Ranjit Sabina S Robinson Camenzind G CG Grace Christy R CR Xia Youlin Y Peng Junmin J Schuetz John D JD

Communications biology 20230624 1

While heme synthesis requires the formation of a potentially lethal intermediate, protoporphyrin IX (PPIX), surprisingly little is known about the mechanism of its toxicity, aside from its phototoxicity. The cellular protein interactions of PPIX might provide insight into modulators of PPIX-induced cell death. Here we report the development of PPB, a biotin-conjugated, PPIX-probe that captures proteins capable of interacting with PPIX. Quantitative proteomics in a diverse panel of mammalian cell ...[more]

PMID: 37355765

Dataset Information

Proteomic Profiling of PPIX-binding proteins between cell types

Dataset's files

Publications

A PPIX-binding probe facilitates discovery of PPIX-induced cell death modulation by peroxiredoxin.

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