Examining the stability of nucleosomal modifications during pull-down affinity purification
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ABSTRACT: Here we investigated the stability of nucleosomal modifications during pull-down affinity purification with HeLa nuclear extracts. Unmodified di-nucleosomes and di-nucleosomes decorated with H3K4me3K9acK14acK18acK23acK27ac, H4K5acK8acK12acK16acK20me2 and incorporating histone variant H2A.Z were incubated with HeLa nuclear extract or buffer alone for 4 hours at 4 degrees Celsius. The relative abundances of nucleosomal modifications were quantified using LC-MS.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Andrey Tvardovskiy
LAB HEAD: Till Bartke
PROVIDER: PXD042823 | Pride | 2023-12-10
REPOSITORIES: Pride
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