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A microbial platform to produce recyclable plastics with customizable physical properties

ABSTRACT: Plastics are one of the most important inventions of the modern era. Unfortunately, they are produced almost exclusively from non-renewable feedstocks (petroleum), and their recycling rates are extremely low, in part due to their chemical composition and the use of composite materials containing plastics that have very different deconstruction properties, making them virtually unrecyclable. Here, we describe the integration of computational, synthetic and polymer chemistry; synthetic biology; and life-cycle and technoeconomic analysis to design and produce highly recyclable plastics (biopolydiketoenimines, bioPDKs) that can be produced from renewable feedstocks and whose recycling properties can be tuned by the chemical substituents placed on one of the key bioPDK monomers, 𝛃-keto-𝛅-lactones (BKDLs). We engineered hybrid polyketide synthases to produce BKDLs with the various chemical substituents and stereochemistries that enable us to tune the conditions under which the resulting bioPDKs can be depolymerized, something that would be nearly impossible to do at scale using synthetic chemistry. These bioPDKs will enable recycling of some of the most difficult to recycle plastics, like composites.

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ORGANISM(S): Streptomyces Albus Subsp. Albus

SUBMITTER: Christopher Petzold

LAB HEAD: Christopher J. Petzold

PROVIDER: PXD042863 | Pride | 2026-05-26

REPOSITORIES: Pride

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Project description:Strains of Pseudomonas putida have emerged as promising biocatalysts for the conversion of sugars and aromatics obtained from lignocellulosic biomass. Understanding the role of carbon catabolite repression (CCR) in these strains is critical to optimizing the conversion of biomass to fuels and chemicals. The functioning of CCR in a P. putida strain, P. putida M2, capable of growing on both hexose and pentose sugars as well as aromatics, was investigated by cultivation experiments, proteomics, and CRISPRi-based gene repression. Strain M2 was able to co-utilize sugars and aromatics simultaneously; however, during co-cultivation with glucose and phenylpropanoid aromatics (p-coumarate and ferulate), intermediates (4-hydroxybenzoate and vanillate) accumulated, and substrate consumption was incomplete. In contrast, xylose-aromatic consumption resulted in transient intermediate accumulation and complete aromatic consumption, while xylose was incompletely consumed. Proteomics analysis of these co- cultivations revealed that glucose exerted stronger repression compared to xylose on the proteins involved in aromatic catabolism. Key glucose (Eda) and xylose (XylX) catabolic proteins were also identified at lower abundance during co-cultivation with aromatics implying simultaneous catabolite repression by sugars and aromatics. Downregulation of crc mediated by CRISPRi led to faster growth and uptake of both glucose and p-coumarate in the CRISPRi strains compared to the control while no difference was observed on xylose + p-coumarate. The increased abundance of the Eda protein and amino acids biosynthesis proteins in the CRISPRi strain further supported these observations. Lastly, small RNAs (sRNAs) sequencing results showed that the levels of CrcY and CrcZ homologs in M2, previously identified as sRNAs involved in CCR in P. putida strains, were lower under strong CCR (glucose + p-coumarate) condition compared to when repression was absent (p-coumarate or glucose only).

			Action	DRS
	Zilong-S-Albus1.MGF	Mgf
	Zilong-S-Albus1.mzid.gz	Mzid
	Zilong-S-Albus1.raw	Raw
	Zilong-S-Albus2.MGF	Mgf
	Zilong-S-Albus2.mzid.gz	Mzid

Dataset Information

A microbial platform to produce recyclable plastics with customizable physical properties

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