Proteomics

Dataset Information

0

Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists


ABSTRACT: Here, we mapped subcellular proteome changes to lysosomes, mitochondrion, EEA1-positive endosomes, and Golgi caused by the NLRP3 inflammasome agonists nigericin and CL097. We identified a number of common disruptions to retrograde trafficking pathways, including COPI and Shiga toxin-related transport, in line with recent studies. We further characterized mouse NLRP3 trafficking throughout its activation using temporal proximity proteomics, which supports a recent model of NLRP3 recruitment to endosomes during inflammasome activation. Collectively, these findings provide additional granularity to our understanding of the molecular events driving NLRP3 activation and serve as a valuable resource for cell biological research.

INSTRUMENT(S): Orbitrap Eclipse, Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Kidney Epithelial Cell, Cell Culture, Macrophage

SUBMITTER: Louis Hollingsworth  

LAB HEAD: J. Wade Harper

PROVIDER: PXD051489 | Pride | 2024-04-23

REPOSITORIES: Pride

Similar Datasets

2024-04-24 | PXD045862 | Pride
2021-11-03 | PXD027728 | Pride
2012-12-01 | E-GEOD-35601 | biostudies-arrayexpress
2014-07-01 | E-GEOD-58959 | biostudies-arrayexpress
2021-12-30 | PXD023877 | Pride
2014-11-27 | E-GEOD-42464 | biostudies-arrayexpress
2023-05-29 | GSE233641 | GEO
2022-11-29 | PXD033497 | Pride
2023-09-07 | PXD041554 | Pride
2023-09-01 | PXD042749 | Pride